Overview

  • Product name

    Anti-Influenza A Virus Nucleoprotein antibody [B247M]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [B247M] to Influenza A Virus Nucleoprotein
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Influenza A
  • Immunogen

    Purified Influenza virus type A (H1N1)

  • Positive control

    • purified Influenza A antigens (H1N1 and H3N2 strains)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    B247M
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization

    Host cell nucleus
  • Database links

    • Alternative names

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    References

    This product has been referenced in:

    • Bolduc M  et al. The quest for a nanoparticle-based vaccine inducing broad protection to influenza viruses. Nanomedicine 14:2563-2574 (2018). Read more (PubMed: 30193813) »
    • Leirs K  et al. Bioassay Development for Ultrasensitive Detection of Influenza A Nucleoprotein Using Digital ELISA. Anal Chem 88:8450-8 (2016). Read more (PubMed: 27487722) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Answer

    Thank you for your response to my further questions. It is very much appreciated.

    When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make sure that the experiments are nicely controlled. Without these details and the exact strains of the virus, it is hard to troubleshoot and to provide sufficient technical support.

    We have not received any similar complaint about ab128193, thus not aware of any QC issues. Currently, we have the same batch in stock your customer has tried. I could offer you a new vial of ab128193 or one vial of any alternative antibodies against the same target protein (Influenza A Virus Nucleoprotein).

    We have the following antibodies which are suitable for Western blot application:

    - https://www.abcam.com/ab43821: Anti-Influenza A Virus Nucleoprotein antibody [9G8]

    - https://www.abcam.com/ab66191: Anti-Influenza A Virus Nucleoprotein antibody [B247M]

    - https://www.abcam.com/ab81126: Anti-Influenza A Virus Nucleoprotein antibody [15]

    Please could you discuss it with your customer and let me know how he/she would like to proceed.

    I look forward to hearing from you soon.

    Read More

    Question
    Answer

    Thank you for contacting us.

    The performa #46714 has been sent.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you for contacting us. The antibodies have different clone number, concentration is different, purity is different, storage buffer percentage is different, and these are tested in different applications. Although these products are against same target but they are different in above mentioned properties. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Answer

    ab66191 has been produced using purified Influenza A virus - the immunogen was not a recombinant protein.

    Read More

    Answer

    Unfortunately we are not aware of any publications for ab66191. However, ab66191 gave a single band against purified Influenza A antigens (H1N1 and H3N2 strains) on the blot (SDS-PAGE, reducing conditions, 1ug antigen/lane). The antibody was tested at 5ug/ml followed by anti-Mouse IgG HRP conjugate.

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