Product nameAnti-Influenza A Virus Nucleoprotein antibody [C43]
See all Influenza A Virus Nucleoprotein primary antibodies
DescriptionMouse monoclonal [C43] to Influenza A Virus Nucleoprotein
Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).
No cross reactivity with influenza B viruses.
Tested applicationsSuitable for: Flow Cyt, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Influenza A
Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
Database link: P69291
Storage instructionsShipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 50% Glycerol, 49% PBS
Concentration information loading...
Purification notesProduced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE
Our Abpromise guarantee covers the use of ab128193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24067955
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||1/300 - 1/1000.|
|ELISA||Use at an assay dependent concentration.|
RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Cellular localizationHost cell nucleus
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34
Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution
Lanes 1 & 6 & 11 : MDCK cells mock infected
Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
Lane 12 : MDCK cells infected H5N1
(A/duck/HK/342/78)collected 3 hrs post-infection
Lane 13 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 9 hrs post-infection
Lane 14 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 24 hrs post-infection
Lane 15 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 48 hrs post-infection
Tubulin was used as loading control.
This product has been referenced in:
- Kupke SY et al. A Novel Type of Influenza A Virus-Derived Defective Interfering Particle with Nucleotide Substitutions in Its Genome. J Virol 93:N/A (2019). Read more (PubMed: 30463972) »
- Leblebici P et al. Encoded particle microfluidic platform for rapid multiplexed screening and characterization of aptamers against influenza A nucleoprotein. Anal Chim Acta 1053:70-80 (2019). Read more (PubMed: 30712571) »