Key features and details
- Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG2a
Product nameAnti-Influenza A Virus Nucleoprotein antibody [C43]
See all Influenza A Virus Nucleoprotein primary antibodies
DescriptionMouse monoclonal [C43] to Influenza A Virus Nucleoprotein
Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).
No cross reactivity with influenza B viruses.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Influenza A
Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
Database link: P69291
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
Purification notesProduced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE
Our Abpromise guarantee covers the use of ab128193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000.|
|ELISA||Use at an assay dependent concentration.|
RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Cellular localizationHost cell nucleus
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34
Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution
Lanes 1 & 6 & 11 : MDCK cells mock infected
Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
Lane 12 : MDCK cells infected H5N1
(A/duck/HK/342/78)collected 3 hrs post-infection
Lane 13 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 9 hrs post-infection
Lane 14 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 24 hrs post-infection
Lane 15 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 48 hrs post-infection
Tubulin was used as loading control.
ab128193 has been referenced in 15 publications.
- Hung SJ et al. Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation. J Biomed Sci 27:17 (2020). PubMed: 31906961
- Yu Y et al. Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways. Virol J 16:163 (2019). PubMed: 31870450
- Kim WJ et al. Pathogenicity of the H1N1 influenza virus enhanced by functional synergy between the NPV100I and NAD248N pair. PLoS One 14:e0217691 (2019). PubMed: 31150476
- Hussain S et al. Segment 2 from influenza A(H1N1) 2009 pandemic viruses confers temperature-sensitive haemagglutinin yield on candidate vaccine virus growth in eggs that can be epistatically complemented by PB2 701D. J Gen Virol 100:1079-1092 (2019). PubMed: 31169484
- Ahn N et al. The Interferon-Inducible Proteoglycan Testican-2/SPOCK2 Functions as a Protective Barrier against Virus Infection of Lung Epithelial Cells. J Virol 93:N/A (2019). PubMed: 31341044