Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)](https://www.abcam.com/ps/products/20/ab20711/Images/ab20711-29983-anti-influenza-b-virus-nucleoprotein-antibody-b017-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)")
Key features and details
- Mouse monoclonal [B017] to Influenza B Virus Nucleoprotein
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Influenza B
- Isotype: IgG2b
Overview
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Product name
Anti-Influenza B Virus Nucleoprotein antibody [B017]
See all Influenza B Virus Nucleoprotein primary antibodies -
Description
Mouse monoclonal [B017] to Influenza B Virus Nucleoprotein -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Influenza B -
Immunogen
Tissue, cells or virus corresponding to Influenza B Influenza B Virus Nucleoprotein. Influenza B/Lee/40 and B/Singapore/-222/79 viruses.
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Positive control
- Recombinant Influenza virus B nucleoprotein or Influenza virus B infected cells. ICC: A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.5
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
>95% pure (SDS-PAGE). Protein G Affinity chromatography. -
Clonality
Monoclonal -
Clone number
B017 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent dilution.
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| WB |
Use at an assay dependent dilution.
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| ICC/IF |
Use at an assay dependent dilution.
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| Notes |
|---|
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ELISA
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. |
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ICC/IF
Use at an assay dependent dilution. |
Target
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Relevance
Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. -
Cellular localization
Nuclear -
Alternative names
- Influenza B Virus NP antibody
- NP antibody
- Nucleocapsid protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)Image from Dauber B et al., PLoS Pathog. 2009 Jun;5(6):e1000473. doi: 10.1371/journal.ppat.1000473. Epub 2009 Jun 12.; Fig 4.; June 12, 2009, PLoS Pathog 5(6): e1000473.Immunofluorescence analysis of A549 cells 8 hours after infection with Influenza B, staining Influenza B Virus Nucleoprotein with ab20711.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab20711 has been referenced in 9 publications.
- Pila-Castellanos I et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274
- Staller E et al. ANP32 Proteins Are Essential for Influenza Virus Replication in Human Cells. J Virol 93:N/A (2019). PubMed: 31217244
- Schmidt N et al. An influenza virus-triggered SUMO switch orchestrates co-opted endogenous retroviruses to stimulate host antiviral immunity. Proc Natl Acad Sci U S A 116:17399-17408 (2019). PubMed: 31391303
- Nogales A et al. Replication-competent fluorescent-expressing influenza B virus. Virus Res 213:69-81 (2016). PubMed: 26590325
- Ran Z et al. Domestic pigs are susceptible to infection with influenza B viruses. J Virol 89:4818-26 (2015). PubMed: 25673727
- Krammer F et al. Assessment of influenza virus hemagglutinin stalk-based immunity in ferrets. J Virol 88:3432-42 (2014). PubMed: 24403585
- Baker SF et al. Influenza A and B virus intertypic reassortment through compatible viral packaging signals. J Virol 88:10778-91 (2014). PubMed: 25008914
- Dauber B et al. Influenza B virus ribonucleoprotein is a potent activator of the antiviral kinase PKR. PLoS Pathog 5:e1000473 (2009). ICC/IF . PubMed: 19521506
- Hai R et al. Influenza B virus NS1-truncated mutants: live-attenuated vaccine approach. J Virol 82:10580-90 (2008). ICC/IF . PubMed: 18768976