Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)

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Key features and details

  • Mouse monoclonal [B017] to Influenza B Virus Nucleoprotein
  • Suitable for: ELISA, WB, ICC/IF
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Influenza B Virus Nucleoprotein antibody [B017]
    See all Influenza B Virus Nucleoprotein primary antibodies

  • Description

    Mouse monoclonal [B017] to Influenza B Virus Nucleoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Influenza B/Lee/40 and B/Singapore/-222/79 viruses.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.50
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    >95% pure (SDS-PAGE). Protein G Affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    B017

  • Myeloma

    P3x63-Ag8.653

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance

    Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • Influenza B Virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Nucleoprotein antibody [B017] (ab20711)Image from Dauber B et al., PLoS Pathog. 2009 Jun;5(6):e1000473. doi: 10.1371/journal.ppat.1000473. Epub 2009 Jun 12.; Fig 4.; June 12, 2009, PLoS Pathog 5(6): e1000473.
      Immunofluorescence analysis of A549 cells 8 hours after infection with Influenza B, staining Influenza B Virus Nucleoprotein with ab20711.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab20711? Please let us know so that we can cite the reference in this datasheet.

    ab20711 has been referenced in 8 publications.

    • Staller E  et al. ANP32 Proteins Are Essential for Influenza Virus Replication in Human Cells. J Virol 93:N/A (2019). PubMed: 31217244
    • Schmidt N  et al. An influenza virus-triggered SUMO switch orchestrates co-opted endogenous retroviruses to stimulate host antiviral immunity. Proc Natl Acad Sci U S A 116:17399-17408 (2019). PubMed: 31391303
    • Nogales A  et al. Replication-competent fluorescent-expressing influenza B virus. Virus Res 213:69-81 (2016). PubMed: 26590325
    • Ran Z  et al. Domestic pigs are susceptible to infection with influenza B viruses. J Virol 89:4818-26 (2015). PubMed: 25673727
    • Krammer F  et al. Assessment of influenza virus hemagglutinin stalk-based immunity in ferrets. J Virol 88:3432-42 (2014). PubMed: 24403585
    • Baker SF  et al. Influenza A and B virus intertypic reassortment through compatible viral packaging signals. J Virol 88:10778-91 (2014). PubMed: 25008914
    • Dauber B  et al. Influenza B virus ribonucleoprotein is a potent activator of the antiviral kinase PKR. PLoS Pathog 5:e1000473 (2009). ICC/IF . PubMed: 19521506
    • Hai R  et al. Influenza B virus NS1-truncated mutants: live-attenuated vaccine approach. J Virol 82:10580-90 (2008). ICC/IF . PubMed: 18768976

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Hi, Could I possibly request a certificate of compliance for ab20711, in particular lot# ****** which we purchased from you and are currently using?

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    Answer

    Thank you for contacting Abcam.

    Please find attached the certificate of compliance for ab20711.

    If there is anything I can help you with, please let me know.

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    Question

    Hi, I'm interested in purchasing "Abcam Mouse monoclonal [B017] to Influenza B Virus Nucleoprotein special catalog number ab68541.  This should be for the 1mL vials.  However, I have been informed that this is no longer available for purchase and to contact you regarding alternatives.  The 100ul vials are too small for our needs and we are looking for a larger aliquot size.  Do you have any suggestions? Thanks,

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    Answer

    Thank you for contacting us. Ab68541 is no longer available in our catalogue however we have other product which has same clone number. The catalogue number is ab20711; https://www.abcam.com/Influenza-B-Virus-Nucleoprotein-antibody-B017-ab20711.html. We also have other products that might also match your requirements. e.g. ab31554, ab47876, ab53936 and ab73440. The ab20711 product is available as 100ul solution which could be diluted 1000 or 500 times to be used in WB or other application. I am sorry I do not understand what you mean by "This should be for the 1mL vials". I you want to buy in bulk then let me know I will inform our goods department to send you 1000ul of the product which will cost you 10 times more of 100ul. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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