Overview

  • Product name

    Anti-Influenza Virus NS1A Binding Protein antibody
    See all Influenza Virus NS1A Binding Protein primary antibodies
  • Description

    Rabbit polyclonal to Influenza Virus NS1A Binding Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen corresponds to a region within amino acids 50-100 of Human Influenza Virus NS1A Binding Protein (NP_006460.2).

  • Positive control

    • HeLa, 293T and NIH/3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab101278 was affinity purified using an epitope specific to Influenza Virus NS1A Binding Protein immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance

    NS1ABP plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats and is known to protect cells from cell death induced by actin destabilization. Interacts with NS/Influenza virus NS1A protein and is believed to mediate the inhibition of its splicing activity. It is usually associated with actin filaments (related to speckle domains) but following Influenza Avirus infection, NS1ABP presents a more diffuse distribution in the nucleoplasm
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Aryl hydrocarbon receptor associated 3 antibody
    • DKFZp686K06216 antibody
    • FLARA 3 antibody
    • FLARA3 antibody
    • HSPC068 antibody
    • IVNS1ABP antibody
    • IVNS1ABP protein antibody
    • KIAA0850 antibody
    • NCX downstream gene 1 antibody
    • ND 1 antibody
    • ND1 antibody
    • NS 1 antibody
    • NS1 antibody
    • NS1 binding protein antibody
    • NS1 binding protein like protein antibody
    • NS1 BP antibody
    • NS1BP antibody
    see all

Images

  • All lanes : Anti-Influenza Virus NS1A Binding Protein antibody (ab101278) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 71 kDa


    Exposure time: 3 minutes
  • Detection of Influenza Virus NS1A Binding Protein by Western Blot of Immunprecipitate.
    ab101278, at 1 µg/ml, staining Influenza Virus NS1A Binding Protein in HeLa whole cell lysate immunoprecipitated using ab101278 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab101278 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3 J2)
Permeabilization
Yes - 0,15% Triton X-100, 5 min
Specification
NIH 3T3 J2
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Simon Boudreault

Verified customer

Submitted Dec 02 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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