Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)
Key features and details
- Rabbit polyclonal to Influenza Virus NS1A Binding Protein
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Influenza Virus NS1A Binding Protein antibody
See all Influenza Virus NS1A Binding Protein primary antibodies -
Description
Rabbit polyclonal to Influenza Virus NS1A Binding Protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 415-600 of Human Influenza Virus NS1A Binding Protein (Q9Y6Y0).
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Positive control
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; Human Huh7 xenograft and Mouse muscle tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab127566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 72 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NS1ABP plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats and is known to protect cells from cell death induced by actin destabilization. Interacts with NS/Influenza virus NS1A protein and is believed to mediate the inhibition of its splicing activity. It is usually associated with actin filaments (related to speckle domains) but following Influenza Avirus infection, NS1ABP presents a more diffuse distribution in the nucleoplasm -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 10625 Human
- Entrez Gene: 117198 Mouse
- Omim: 609209 Human
- SwissProt: Q9Y6Y0 Human
- SwissProt: Q920Q8 Mouse
- Unigene: 497183 Human
- Unigene: 33764 Mouse
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Alternative names
- Aryl hydrocarbon receptor associated 3 antibody
- DKFZp686K06216 antibody
- FLARA 3 antibody
see all
Images
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Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)Anti-Influenza Virus NS1A Binding Protein antibody (ab127566) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)ab127566, at 1/500 dilution, staining Influenza Virus NS1A Binding Protein in Human Huh7 xenograft tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)ab127566, at 1/500 dilution, staining Influenza Virus NS1A Binding Protein in paraffin-embedded Mouse muscle tissue by Immunohistochemistry.
Protocols
References (0)
ab127566 has not yet been referenced specifically in any publications.
