Product nameAnti-ING1 antibody [EPR6788]
See all ING1 primary antibodies
DescriptionRabbit monoclonal [EPR6788] to ING1
Tested applicationsSuitable for: IHC-Fr, WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human ING1. (Uniprot: Q9UK53)
- HepG2 lysate, HeLa lysate, MCF-7 lysate, Jurkat lysate, C6 lysate, RAW264.7 lysate, PC-12 lysate and NIH/3T3 lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 26100219|
|WB||1/1000 - 1/10000. Predicted molecular weight: 47 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.
Tissue specificityIsoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines.
Involvement in diseaseDefects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
Sequence similaritiesBelongs to the ING family.
Contains 1 PHD-type zinc finger.
- Information by UniProt
- 2610028J21Rik antibody
- AA407184 antibody
- AI875420 antibody
All lanes : Anti-ING1 antibody [EPR6788] (ab124893) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : MCF-7 lysate
Lane 4 : Jurkat lysate
Lane 5 : C6 lysate
Lane 6 : RAW 264.7 lysate
Lane 7 : PC-12 lysate
Lane 8 : NIH/3T3 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa
Flow Cytometric analysis of ab124893, at 1/10 dilution, staining ING1 in permeabilised NIH/3T3cells (red). Isotype control (Green).
This product has been referenced in:
- Yan H & Wu A FOXO1 is crucial in glioblastoma cell tumorigenesis and regulates the expression of SIRT1 to suppress senescence in the brain. Mol Med Rep 17:2535-2542 (2018). Read more (PubMed: 29207098) »
- Kim SW et al. Latent progenitor cells as potential regulators for tympanic membrane regeneration. Sci Rep 5:11542 (2015). IHC-Fr ; Rat . Read more (PubMed: 26100219) »