Anti-ING2 antibody (ab2643)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ING2 antibody
See all ING2 primary antibodies -
Description
Goat polyclonal to ING2 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: DKSTEKTKKDRRSR, corresponding to C terminal amino acids 267-280 of Human ING2.
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Positive control
- HepG2 lysate
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General notes
Principal Names - ING1L; inhibitor of growth family, member 1-like; p33ING2; inhibitor of growth 1-like Official Gene Symbol - ING1L GenBank Accession Number – NP_001555 LocusLink ID - 3622 (human) Gene Ontology terms - chromatin binding; signal transduction; chromosome
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab2643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32.8 kDa). | |
| ICC/IF | Use at an assay dependent dilution. |
Target
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Function
Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs). -
Tissue specificity
Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues. -
Sequence similarities
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
Domain
The PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P). -
Cellular localization
Nucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3622 Human
- Omim: 604215 Human
- SwissProt: Q9H160 Human
- Unigene: 107153 Human
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Alternative names
- ING 1 like tumor suppressor protein antibody
- ING 1L antibody
- ING 2 antibody
see all
Images
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Western blot - Anti-ING2 antibody (ab2643)Anti-ING2 antibody (ab2643) at 2 µg/ml + HepG2 lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 32.8 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Datasheets and documents
References
This product has been referenced in:
- Pedeux R et al. ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. Mol Cell Biol 25:6639-48 (2005). Read more (PubMed: 16024799) »
