Overview

  • Product name
    Anti-ING2 antibody
    See all ING2 primary antibodies
  • Description
    Goat polyclonal to ING2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: DKSTEKTKKDRRSR, corresponding to C terminal amino acids 267-280 of Human ING2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HepG2 lysate
  • General notes
    Principal Names - ING1L; inhibitor of growth family, member 1-like; p33ING2; inhibitor of growth 1-like Official Gene Symbol - ING1L GenBank Accession Number – NP_001555 LocusLink ID - 3622 (human) Gene Ontology terms - chromatin binding; signal transduction; chromosome

Properties

Applications

Our Abpromise guarantee covers the use of ab2643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32.8 kDa).Can be blocked with Human ING2 peptide (ab22925).
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
  • Tissue specificity
    Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
  • Sequence similarities
    Belongs to the ING family.
    Contains 1 PHD-type zinc finger.
  • Domain
    The PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
  • Cellular localization
    Nucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • ING 1 like tumor suppressor protein antibody
      • ING 1L antibody
      • ING 2 antibody
      • ING1 like tumor suppressor protein antibody
      • ING1L antibody
      • ING1Lp antibody
      • ING2 antibody
      • ING2_HUMAN antibody
      • Inhibitor of growth 1 like antibody
      • Inhibitor of growth 1 like protein antibody
      • Inhibitor of growth 1-like protein antibody
      • Inhibitor of growth family member 1 like antibody
      • Inhibitor of growth family member 2 antibody
      • Inhibitor of growth protein 2 antibody
      • p32 antibody
      • p33 ING2 antibody
      • p33ING2 antibody
      see all

    Images

    • Western blot - Anti-ING2 antibody (ab2643)
      Western blot - Anti-ING2 antibody (ab2643)
      Anti-ING2 antibody (ab2643) at 2 µg/ml + HepG2 lysate (RIPA buffer, 35µg total protein per lane).

      Predicted band size: 32.8 kDa
      Observed band size: 30 kDa (why is the actual band size different from the predicted?)



      Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

    References

    This product has been referenced in:
    • Pedeux R  et al. ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. Mol Cell Biol 25:6639-48 (2005). Read more (PubMed: 16024799) »

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