Anti-ING2 antibody (ab2643)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-ING2 antibody
See all ING2 primary antibodies -
DescriptionGoat polyclonal to ING2
-
Host speciesGoat
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide: DKSTEKTKKDRRSR, corresponding to C terminal amino acids 267-280 of Human ING2.
-
Positive control
- HepG2 lysate
-
General notesPrincipal Names - ING1L; inhibitor of growth family, member 1-like; p33ING2; inhibitor of growth 1-like Official Gene Symbol - ING1L GenBank Accession Number – NP_001555 LocusLink ID - 3622 (human) Gene Ontology terms - chromatin binding; signal transduction; chromosome
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab2643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32.8 kDa).Can be blocked with Human ING2 peptide (ab22925). | |
| ICC/IF | Use at an assay dependent dilution. |
Target
-
FunctionSeems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
-
Tissue specificityWidely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
-
Sequence similaritiesBelongs to the ING family.
Contains 1 PHD-type zinc finger. -
DomainThe PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
-
Cellular localizationNucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 3622 Human
- Omim: 604215 Human
- SwissProt: Q9H160 Human
- Unigene: 107153 Human
-
Alternative names
- ING 1 like tumor suppressor protein antibody
- ING 1L antibody
- ING 2 antibody
see all
Images
-
Western blot - Anti-ING2 antibody (ab2643)Anti-ING2 antibody (ab2643) at 2 µg/ml + HepG2 lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 32.8 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Datasheets and documents
References
This product has been referenced in:
- Pedeux R et al. ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. Mol Cell Biol 25:6639-48 (2005). Read more (PubMed: 16024799) »
