Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4597-5] to ING2
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-ING2 antibody [EPR4597-5]
See all ING2 primary antibodies
DescriptionRabbit monoclonal [EPR4597-5] to ING2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human ING2. The exact sequence is proprietary.
Database link: Q9H160
- Hela, HT-1080 and Saos-2 cell lysates; Human ING2 recombinant protein.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 33 kDa.|
FunctionSeems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
Tissue specificityWidely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
Sequence similaritiesBelongs to the ING family.
Contains 1 PHD-type zinc finger.
DomainThe PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
Cellular localizationNucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
- Information by UniProt
- ING 1 like tumor suppressor protein antibody
- ING 1L antibody
- ING 2 antibody
All lanes : Anti-ING2 antibody [EPR4597-5] (ab174305) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : HT-1080 cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa
Anti-ING2 antibody [EPR4597-5] (ab174305) at 1/1000 dilution + ING2 recombinant protein (10ng)
Predicted band size: 33 kDa
ab174305 has not yet been referenced specifically in any publications.