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BATCH NUMBER 63946 DESCRIPTION OF THE PROBLEM No any Band. SAMPLE Cell Lysate PRIMARY ANTIBODY ab3714 SECONDARY ANTIBODY ABcam/Rabbit anti Goat IgG HRP DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Cell Lysate SAMPLE PREPARATION PBS Buffer TRANSFER AND BLOCKING CONDITIONS 0.5% milk in PBS HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? No
Asked on Oct 29 2004
Thank you for your enquiry. We would like to emphasize the fact that this is a Fast track antibody and it has only been assayed by ELISA against 0.1µg of the immunizing peptide. It has not been tested for Western blot analysis at all. We have read through the completed questionnaire; however we still need the following information. Unfortunately, the details you have provided are not enough for us to be able to identify the source of the problem. What type of samples your customer has tested (species, cells, tissue, recombinant protein etc)? How was the cell lysate prepared? How much protein was loaded onto the gel? At what dilution the antibody was tested? Has the customer tested the detection system? How about the protein transfer? What did he/she use as positive control? We look forward to hearing from you soon.
Answered on Nov 01 2004