Overview

  • Product name
  • Description
    Goat polyclonal to ING5
  • Host species
    Goat
  • Specificity
    This antibody has only been used to detect purified ING5 and has not been used with whole cell lysates.
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    CGLKKGRGQKEKRGS

    , corresponding to amino acids 127-140 of Human ING5.

  • General notes


    ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.

Properties

Applications

Our Abpromise guarantee covers the use of ab3716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 28 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 21177815

Target

  • Function
    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator.
  • Sequence similarities
    Belongs to the ING family.
    Contains 1 PHD-type zinc finger.
  • Domain
    The PHD-type zinc finger mediates the binding to H3K4me3.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700001C14Rik antibody
    • 1700027H23Rik antibody
    • 1810018M11Rik antibody
    • FLJ23842 antibody
    • ING 5 antibody
    • Ing5 antibody
    • ING5_HUMAN antibody
    • Inhibitor of growth family member 5 antibody
    • Inhibitor of growth protein 5 antibody
    • p28 ING 5 antibody
    • p28 ING5 antibody
    • p28ING5 antibody
    see all

Images

  • ING5 antibody (ab3716) was used at 1:500 and detected using chemiluminescence reagent. Exposure 5 minutes.
    Lane 1: Mock purification
    Lane 2: Purified TAP tagged ING4 protein
    Lane 3: Purified TAP tagged ING5 from Hela nuclear exracts.

    This WB shows the ING5 Ab working on purified material.  ab3716 does not recognize ING4 on the same membrane which shows the antibody is specific as ING4 and ING5 are very similar proteins.

    ING5 is detected at 44kD due to the TAP tag (predicted size is 28kD).

    ING5 antibody (ab3716) was used at 1:500 and detected using chemiluminescence reagent. Exposure 5 minutes.

    Lane 1: Mock purification
    Lane 2: Purified TAP tagged ING4 protein
    Lane 3: Purified TAP tagged ING5 from Hela nuclear exracts.

    This WB shows the ING5 Ab working on purified material. ab3716 does not recognize ING4 on the same membrane which shows the antibody is specific as ING4 and ING5 are very similar proteins.

    ING

References

This product has been referenced in:
  • Zhang X  et al. ING5 is a Potential Target for Osteosarcoma Therapy. Technol Cancer Res Treat 17:1533033818762680 (2018). Read more (PubMed: 29528777) »
  • Pardo M  et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). Read more (PubMed: 28811661) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

I had forgotten that yesterday was a holiday for our UK office (May day) and so you should expect to receive it by Thursday. I just found out this information: This antibody was released as a fully characterized antibody when it should have been released as a "Fast Track" antibody. Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. The antibody is considered to still be in development and was released early due to public interest. I apologize for this mistake and the online datasheet has been updated to reflect this antibody's status. Your feedback regarding this antibody, negative or positive, is greatly appreciated. If you are not happy with how this antibody works for you I will happily give you a full refund. If you do find the antibody to work, we will offer you $300 in conference travel money in exchange for positive data (such as a WB image). Please do let me know how the new vial of ab3716 works out for you and if you have any more questions, please contact me again.

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Answer

My feeling as well is that the batch is off or your particular vial went off during shipping, etc. We have had no other complaints concerning this particular antibody. We have a new batch in stock and I can send you a vial free of charge if you are interested. Please let me know what you would like to do.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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