Recombinant Anti-ING5 antibody [EPR23930-1] (ab259904)

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Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23930-1] to ING5
Suitable for: IP, ICC, Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-ING5 antibody [EPR23930-1]
See all ING5 primary antibodies
Description

Rabbit monoclonal [EPR23930-1] to ING5
Host species

Rabbit
Tested applications

Suitable for: IP, ICC, Flow Cyt, WBmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human ING5 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q8WYH8
Positive control
- WB: HEK-293T, HeLa, A549, MCF7, MDA-MB-231 and NIH/3T3 whole cell lysates; His-tagged human ING5 recombinant protein. ICC: HEK-293T and NIH/3T3 cells. Flow cyt: HEK-293T and NIH/3T3 cells. IP: MCF7 and NIH/3T3 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23930-1
Isotype

IgG
Research areas

Alternative Versions
- Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Our Abpromise guarantee covers the use of ab259904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		1/30.
ICC		1/1000.
Flow Cyt		1/500.
WB		1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).

Application notes

Is unsuitable for IHC-P.

Function

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator.
Sequence similarities

Belongs to the ING family.
Contains 1 PHD-type zinc finger.
Domain

The PHD-type zinc finger mediates the binding to H3K4me3.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q8WYH8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 84289 Human
- Entrez Gene: 66262 Mouse
- Entrez Gene: 363292 Rat
- Omim: 608525 Human
- SwissProt: Q8WYH8 Human
- SwissProt: Q9D8Y8 Mouse
- Unigene: 529172 Human
- Unigene: 64065 Mouse
- Unigene: 214030 Rat
see all
Alternative names
- 1700001C14Rik antibody
- 1700027H23Rik antibody
- 1810018M11Rik antibody
- FLJ23842 antibody
- ING 5 antibody
- Ing5 antibody
- ING5_HUMAN antibody
- Inhibitor of growth family member 5 antibody
- Inhibitor of growth protein 5 antibody
- p28 ING 5 antibody
- p28 ING5 antibody
- p28ING5 antibody
see all

Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904)

All lanes : Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 32 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane1-2: 10 secondsLane3-4: 15 seconds.
Immunocytochemistry - Anti-ING5 antibody [EPR23930-1] (ab259904)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling ING5 with ab259904 at 1/1000 (0.482 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in HEK-293T cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904)

All lanes : Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer:5% NFDM/TBST.

Exposure times: Lane1-2: 7.5 seconds; Lane3: 70 seconds.
Immunocytochemistry - Anti-ING5 antibody [EPR23930-1] (ab259904)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling ING5 with ab259904 at 1/1000 (0.482 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Flow Cytometry - Anti-ING5 antibody [EPR23930-1] (ab259904)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling ING5 with ab259904 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (ab259904)

ING5 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab259904 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259904 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab259904 IP in MCF7 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259904 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Flow Cytometry - Anti-ING5 antibody [EPR23930-1] (ab259904)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ING5 with ab259904 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (ab259904)

ING5 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab259904 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259904 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug

Lane 2: ab259904 IP in NIH/3T3 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259904 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904)

All lanes : Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

Lane 1 : His-tagged human ING5 recombinant protein, 20 ng
Lane 2 : His-tagged human ING4 recombinant protein, 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 28 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane1: 8 seconds; Lane2: 3 minutes
Anti-ING5 antibody [EPR23930-1] (ab259904)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab259904? Please let us know so that we can cite the reference in this datasheet.

ab259904 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-ING5 antibody [EPR23930-1] (ab259904)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (0)

Customer reviews and Q&As