Overview

  • Product name
    Anti-Inhibin alpha antibody [4A2F2]
    See all Inhibin alpha primary antibodies
  • Description
    Mouse monoclonal [4A2F2] to Inhibin alpha
  • Host species
    Mouse
  • Specificity
    This antibody is specific for Inhibin alpha.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-P, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Ni-NTA purified truncated recombinant Inhibin alpha expressed in E. Coli strain BL21 (DE3)

  • Positive control
    • Human lymphoid tissue, human ovary tumor tissue and human testicle tumor tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    4A2F2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
IHC-Fr 1/200 - 1/1000.

Target

  • Function
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Tissue specificity
    Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    modifications
    Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A inhibin subunit antibody
    • A inhibin subunit precursor antibody
    • AW55578 antibody
    • IHA antibody
    • inhA antibody
    • INHA_HUMAN antibody
    • Inhibin alpha chain antibody
    • Inhibin alpha chain precursor antibody
    • Inhibin alpha subunit antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human lymphoid tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded human testicle tumor tissue showing cytoplasmic/ membrane localization using ab47720 at a 1/500 dilution, with DAB staining.
  • ab47720 at 1/1000 dilution staining Inhibin alpha in human Hela cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary show green staining in image while actin filaments have been labelled red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.

  • Overlay histogram showing HeLa cells stained with ab47720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47720, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

Gentaur is not an official distributor of Abcam so the price will be the same as seen on website. 1 vial of ab47720 will cost you 235 GBP with £16 delivery charges in UK and €380 with €21 in Germany.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your email. Ab4241 is raised against Inhibin beta A chain and ab47720 is raised against Inhibin alpha cahin. Theoretically these antibodies can be used in sELISA experiment. I am however unable to confirm because we haven't tested these antibodies together. We do not have data to prove the assumption of using this abs together. You may need to optimize the protocol yourselves. TheInhibin beta A chain and Inhibin alpha protein sequence does not match so the antibodies against these proteins can be used in sELISA. Please also be advised that ab4241 and ab47720 are not conjugated antibodies; any of these can be used as detection or capture ab however you will need a tertiary conjugated antibody for detection. Now if you are planning to use ab4241 and ab47720 please be aware that both of these are mouse monoclonal; the tertiary anti mouse antibody will bind to both capture and detections ab thereforewill givehigh background intensity. This situation can be overcome by using one rabbit and other mouse antibody. I suggest using ab93847, ab97705 or ab113489 as substitution for ab4241 or ab35106, ab81234 as subs for ab47720. Finally, if you choose 2 antibodies taking in consideration of the above information I sent; I can then provide you2 Abreview discount codes for using these antibodies together in sELISA. You can get 2 antibodies freeof charge in your next purchase with these discount codes. To activate the discount codes you have to use the products together thereby submitting an Abreview. Please click the link below for further update; https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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Answer

I have received your inquiry and have started working on it. I am seeking more information from lab regarding your inquiry. I will get back to you with the information soon. Many thanks for having patience!

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Answer

Thank you for contacting us. The product ab119449 is an Inhibin beta A protein however the antibodies are ab105927 (4A2F2)and ab47720 (4E2) are anti Inihibin alpha so these antibodies cannot used in conjunction with ab119449. You may however consider using ab4241 or ab93847 with ab119449. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and your interest in our products. Unit size, price, availability, tested applications and species can be found on the on-line datasheets: https://www.abcam.com/index.html?datasheet=4241 https://www.abcam.com/index.html?datasheet=47720 https://www.abcam.com/index.html?datasheet=56057 https://www.abcam.com/index.html?datasheet=81234 I can confirm that these antibodies have been tested for ELISA but NOT in Sandwich ELISA (sELISA). We have no information if they work together in pairs since no data available yet for sELISA. However, they should work in indirect ELISA system with suitable compatible secondary antibodies. I have conducted search and currently we can't offer antibodies for sELISA for these two targets: - Inhibin beta A - Inhibin alpha. If you need any further assistance in the future, please do not hesitate to contact me.      

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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