Recombinant Anti-Inhibin alpha antibody [EPY2782] - BSA and Azide free (ab226844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPY2782] to Inhibin alpha - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Inhibin alpha antibody [EPY2782] - BSA and Azide free
See all Inhibin alpha primary antibodies -
Description
Rabbit monoclonal [EPY2782] to Inhibin alpha - BSA and Azide free -
Host species
Rabbit -
Specificity
Note: 20/23 kDa bands consist of the mature alpha chain. 50/53 kDa bands are the entire proprotein.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human adrenal gland and placenta lysate; HeLa and LNCaP cell lysates.
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General notes
ab226844 is the carrier-free version of ab81322.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPY2782 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
Tissue specificity
Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsProteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3623 Human
- Entrez Gene: 16322 Mouse
- Entrez Gene: 24504 Rat
- Omim: 147380 Human
- SwissProt: P05111 Human
- SwissProt: Q04997 Mouse
- SwissProt: P17490 Rat
- Unigene: 407506 Human
see all -
Alternative names
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
see all
Images
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All lanes : Anti-Inhibin alpha antibody [EPY2782] (ab81322) at 1/2000 dilution
Lane 1 : Human adrenal gland lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Human placenta lysate
Lane 4 : LNCaP (Human prostate cancer cell line) cell lysate
Secondary
All lanes : HRP goat anti rabbit at 1/2000 dilution
Predicted band size: 40 kDaNote: the 50 kDa band corresponds to the entire proprotein.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81322).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab226844 has not yet been referenced specifically in any publications.