Key features and details
- Rabbit polyclonal to Inhibin alpha - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Inhibin alpha antibody - N-terminal
See all Inhibin alpha primary antibodies
DescriptionRabbit polyclonal to Inhibin alpha - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Frog
Synthetic peptide within Human Inhibin alpha aa 200-300 (N terminal). The exact sequence is proprietary.
Database link: P05111
- WB: HeLa cell lysate. IHC-P: Human testis tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab231459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary antibody incubation for 30 minutes at room temperature.
|WB||1/25. Predicted molecular weight: 40 kDa.
Primary antibody incubation for 1 hour at room temperature.
FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Tissue specificityOriginally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
Sequence similaritiesBelongs to the TGF-beta family.
modificationsProteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
- Information by UniProt
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
Formalin-fixed, paraffin-embedded human testis tissue stained for Inhibin alpha using ab231459 at 1/100 dilution in immunohistochemical analysis.
Anti-Inhibin alpha antibody - N-terminal (ab231459) at 1/25 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 40 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231459 has not yet been referenced specifically in any publications.