Anti-Inhibin alpha antibody [R1] (ab14087)
Key features and details
- Mouse monoclonal [R1] to Inhibin alpha
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Inhibin alpha antibody [R1]
See all Inhibin alpha primary antibodies -
Description
Mouse monoclonal [R1] to Inhibin alpha -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Inhibin alpha aa 233-264. Note that this peptide corresponds to the N-terminus of the mature region of the human inhibin alpha subunit.
Sequence:STPLMSWPWSPSALRLLQRPPEEPAAHANCHR
Database link: P05111 -
Positive control
- human ovary, testis, placenta
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General notes
Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Primary antibody notes
Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours. -
Clonality
Monoclonal -
Clone number
R1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval. |
Notes |
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IHC-P
1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval. |
Target
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Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
Tissue specificity
Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsProteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3623 Human
- Omim: 147380 Human
- SwissProt: P05111 Human
- Unigene: 407506 Human
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Alternative names
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of paraffin embedded human ovary tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue (showing sertoli cells, leydig cells and spermatogonia) labeling inhibin alpha with ab14087.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)
Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab14087 has been referenced in 4 publications.
- Kusumoto S et al. An immature inhibin-a-expressing subpopulation of ovarian clear cell carcinoma cells is related to an unfavorable prognosis. Cancer Med 10:1485-1500 (2021). PubMed: 33611864
- Walton CC et al. Primary neurons can enter M-phase. Sci Rep 9:4594 (2019). PubMed: 30872646
- Xu Y et al. Cystic and solitary nodular pulmonary metastases in a patient with low-grade endometrial stromal sarcoma: A case report and literature review. Oncol Lett 18:1133-1144 (2019). PubMed: 31423173
- Chen J et al. Computed tomography and magnetic resonance imaging features of ovarian fibrothecoma. Oncol Lett 14:1172-1178 (2017). PubMed: 28693292