Overview

  • Product name

    Anti-Inhibin alpha antibody [SP323]
    See all Inhibin alpha primary antibodies
  • Description

    Rabbit monoclonal [SP323] to Inhibin alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Inhibin alpha (internal sequence).
    Database link: P05111

  • Positive control

    • IHC-P: Human stomach carcinoma, testis, adrenal gland, and Mouse testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP323
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.

Target

  • Function

    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Tissue specificity

    Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Post-translational
    modifications

    Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A inhibin subunit antibody
    • A inhibin subunit precursor antibody
    • AW55578 antibody
    • IHA antibody
    • inhA antibody
    • INHA_HUMAN antibody
    • Inhibin alpha chain antibody
    • Inhibin alpha chain precursor antibody
    • Inhibin alpha subunit antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach adenocarcinoma tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human testis tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

References

ab224798 has not yet been referenced specifically in any publications.

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