Product nameAnti-Inhibin beta A antibody
See all Inhibin beta A primary antibodies
DescriptionRabbit polyclonal to Inhibin beta A
Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Inhibin beta A (N terminal). A synthetic peptide corresponding to N-terminal residues of human INHBA (Inhibin beta A chain precursor)
Database link: P08476
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab56057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- Activin beta-A chain antibody
- EDF antibody
- Erythroid differentiation factor antibody
Anti-Inhibin beta A antibody (ab56057) at 1/1000 dilution + Human COV434 whole cell lysate at 60 µg
IRDye® 680LT-conjugated goat anti-rabbit IgG polyclonal at 1/25000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
ab56057 staining Inhibin in human testicular cancer (seminoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked for 20 minutes at 26°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.
ab56057 staining Inhibin beta A in human COV434 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with ethanol and blocked for 3 hours at 26°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 21°C. A IRDye® 800CW-conjugated goat anti-rabbit IgG (H+L) polyclonal (1/15000) was used as the secondary antibody.
ab56057 has been referenced in 3 publications.
- Lyu S et al. INHBA upregulation correlates with poorer prognosis in patients with esophageal squamous cell carcinoma. Cancer Manag Res 10:1585-1596 (2018). PubMed: 29950896
- Nagaraja AS et al. Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens. JCI Insight 2:N/A (2017). PubMed: 28814667
- Locci M et al. Activin A programs the differentiation of human TFH cells. Nat Immunol 17:976-84 (2016). PubMed: 27376469