Recombinant
RabMAb

Recombinant Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)

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Overview

  • Product name

    Anti-Inhibin beta A antibody [EPR2785(2)]
    See all Inhibin beta A primary antibodies

  • Description

    Rabbit monoclonal [EPR2785(2)] to Inhibin beta A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Inhibin beta A aa 50-150. The exact sequence is proprietary.
    Database link: P08476
    (Peptide available as ab185871)

  • Positive control

    • Recombinant Human Inhibin beta A protein
      Fetal brain, fetal liver and mouse brain lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.93 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2785(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

    • Sequence similarities

      Belongs to the TGF-beta family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Activin beta-A chain antibody
      • EDF antibody
      • Erythroid differentiation factor antibody
      • Erythroid differentiation protein antibody
      • Follicle stimulating hormone releasing protein antibody
      • FRP antibody
      • FSH releasing protein antibody
      • INHBA antibody
      • INHBA_HUMAN antibody
      • Inhibin beta A chain antibody
      • Inhibin beta A subunit antibody
      • Inhibin, beta 1 antibody
      • Inhibin, beta A (activin A, activin AB alpha polypeptide) antibody
      see all

    Images

    • Other - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
      Other - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
      Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
      Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958) at 1/1000 dilution + Human Inhibin beta A recombinant protein at 0.001 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
      Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
      All lanes : Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : Mouse brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Wu Y  et al. Autocrine transforming growth factor-ß/activin A-Smad signaling induces hepatic progenitor cells undergoing partial epithelial-mesenchymal transition states. Biochimie 148:87-98 (2018). Read more (PubMed: 29544731) »
    See 1 Publication for this product

    Publishing research using ab128958? Please let us know so that we can cite the reference in this datasheet.

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