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Signal Transduction Growth Factors/Hormones Hormones
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RecombinantRabMAb

Recombinant Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)

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Western blot - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
  • Western blot - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
  • OI-RD Scanning - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
  • Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2788(2)] to Inhibin beta B
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Primary
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Protein
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Recombinant Human Inhibin beta B protein (ab158764)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Inhibin beta B antibody [EPR2788(2)]
    See all Inhibin beta B primary antibodies
  • Description

    Rabbit monoclonal [EPR2788(2)] to Inhibin beta B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Inhibin beta B aa 350 to the C-terminus.
    Database link: P09529
    (Peptide available as ab174743)

  • Positive control

    • Mouse brain and Rat brain lysates; Human Inhibin beta B recombinant protein (mature form: 17 kDa)
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.78 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2788(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Neuroscience
    • Endocrine system
    • Hypothalamic pituitary adrenal axis

Associated products

  • Alternative Versions

    • Anti-Inhibin beta B antibody [EPR2788(2)] - BSA and Azide free (ab226845)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
  • Recombinant Protein

    • Recombinant Human Inhibin beta B protein (ab158764)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52, 17 kDa (predicted molecular weight: 45 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52, 17 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P09529 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3625 Human
      • Entrez Gene: 16324 Mouse
      • Entrez Gene: 25196 Rat
      • Omim: 147390 Human
      • SwissProt: P09529 Human
      • SwissProt: Q04999 Mouse
      • SwissProt: P17491 Rat
      • Unigene: 1735 Human
      • Unigene: 3092 Mouse
      • Unigene: 35074 Rat
      see all
    • Alternative names

      • Activin beta B chain antibody
      • Activin beta-B chain antibody
      • INHBB antibody
      • INHBB_HUMAN antibody
      • Inhibin beta 2 antibody
      • Inhibin beta B chain antibody
      • Inhibin beta B subunit antibody
      see all

    Images

    • Western blot - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      Western blot - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      All lanes : Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      Western blot - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944) at 1/1000 dilution + Human Inhibin beta B recombinant protein (mature form:
      17 kDa) (10 ng)

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • OI-RD Scanning - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      OI-RD Scanning - Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)
      Anti-Inhibin beta B antibody [EPR2788(2)] (ab128944)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab128944? Please let us know so that we can cite the reference in this datasheet.

    ab128944 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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