Overview

  • Product name

  • Description

    Rabbit polyclonal to INO80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human INO80 aa 1500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab123818)

  • Positive control

    • This antibody gave a positive signal in human brain tissue lysate and HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.
  • Tissue specificity

    According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 DBINO domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain

    The DBINO region is involved in binding to DNA.
  • Cellular localization

    Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA helicase INO80 antibody
    • hINO80 antibody
    • homolog of yeast INO80 antibody
    • INO80 antibody
    • INO80 complex subunit A antibody
    • INO80 homolog antibody
    • INO80_HUMAN antibody
    • INO80A antibody
    • INOC1 antibody
    • KIAA1259 antibody
    • Putative DNA helicase INO80 complex homolog 1 antibody
    see all

Images

  • All lanes : Anti-INO80 antibody (ab105451) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 177 kDa
    Observed band size: 177 kDa
    Additional bands at: 32 kDa, 64 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • ICC/IF image of ab105451 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105451, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2, MCF7 cells at 1µg/ml.

References

This product has been referenced in:

  • Rhee S  et al. Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease. Nat Commun 9:368 (2018). Read more (PubMed: 29371594) »
  • Runge JS  et al. Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide. G3 (Bethesda) 8:1095-1102 (2018). Read more (PubMed: 29432129) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us. Based on the sequence homology with the immunogen used to generate this antibody there is low likelihood of reactivity with S.cerevisiae.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse embryo OCT section e16.5)
Specification
Mouse embryo OCT section e16.5
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Andrew Mckay

Verified customer

Submitted Oct 25 2012

Answer

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.
Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab105451.
When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : www.abcam.com/ab9385.
Should the suggestions not improve the results, please do let me know.
In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.
I hope this information is helpful, and I thank you for your cooperation.

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Question
Answer



I am very pleased to hear you would like to accept our offer and test ab105451 in mouse. This code will give you a discount off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you very much for your interest in ab105451. We have tested and would guarantee this antibody to work in WB and ICC/IF using human samples.

Would your KO be in mouse? We don't have sample sizes of our products because we carry so many and can't store multiple vial sizes. Instead, we offer a testing discount program to trial antibodies in untested species or applications.

To our knowledge, ab105451 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab105451 now, test it inmouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody of your following order from Abcam.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab105451 in mouse. I will then send a discount code. This code must be issued before purchasing ab105451 so please wait for my reply before ordering.

2. Purchase ab105451 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab105451 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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