Anti-INO80 antibody (ab118787)

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Western blot - Anti-INO80 antibody (ab118787)
  • Immunoprecipitation - Anti-INO80 antibody (ab118787)

Key features and details

  • Rabbit polyclonal to INO80
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-INO80 antibody
    See all INO80 primary antibodies

  • Description

    Rabbit polyclonal to INO80

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat

  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1506-1556 of Human INO80 (NP_060023.1).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab118787 was affinity purified using an epitope specific to INO80 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 176 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.

  • Tissue specificity

    According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.

  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 DBINO domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Domain

    The DBINO region is involved in binding to DNA.

  • Cellular localization

    Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.
  • Information by UniProt

  • Database links

    • Alternative names

      • DNA helicase INO80 antibody
      • hINO80 antibody
      • homolog of yeast INO80 antibody
      • INO80 antibody
      • INO80 complex subunit A antibody
      • INO80 homolog antibody
      • INO80_HUMAN antibody
      • INO80A antibody
      • INOC1 antibody
      • KIAA1259 antibody
      • Putative DNA helicase INO80 complex homolog 1 antibody
      see all

    Images

    • Western blot - Anti-INO80 antibody (ab118787)
      Western blot - Anti-INO80 antibody (ab118787)
      All lanes : Anti-INO80 antibody (ab118787) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 176 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-INO80 antibody (ab118787)
      Immunoprecipitation - Anti-INO80 antibody (ab118787)
      Detection of INO80 in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118787 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References (8)

    Publishing research using ab118787? Please let us know so that we can cite the reference in this datasheet.

    ab118787 has been referenced in 8 publications.

    • Prendergast L  et al. Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability. Nat Commun 11:4534 (2020). PubMed: 32913330
    • Lee HS  et al. BAP1 promotes stalled fork restart and cell survival via INO80 in response to replication stress. Biochem J 476:3053-3066 (2019). PubMed: 31657441
    • Lee SA  et al. INO80 haploinsufficiency inhibits colon cancer tumorigenesis via replication stress-induced apoptosis. Oncotarget 8:115041-115053 (2017). PubMed: 29383140
    • Lee BH & Stallcup MR Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes. J Biol Chem 292:9320-9334 (2017). PubMed: 28381557
    • Lu X  et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). PubMed: 28598414
    • Mendiratta S  et al. Interaction of the Chromatin Remodeling Protein hINO80 with DNA. PLoS One 11:e0159370 (2016). PubMed: 27428271
    • Alatwi HE & Downs JA Removal of H2A.Z by INO80 promotes homologous recombination. EMBO Rep 16:986-94 (2015). PubMed: 26142279
    • Seo J  et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24163101

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    ChIP abreview for Anti-INO80 antibody

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Human Umbilical Vein Cells (HUVEC))
    Negative control
    IgG was used as negative control in ChIP.
    Specification
    Human Umbilical Vein Cells (HUVEC)
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaledehyde final concent
    Positive control
    Transcriptional start site (TSS) of specified genes.
    Read More

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    Verified customer

    Submitted Oct 16 2017

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