Application
ChIP
Sample
Human Cell lysate - whole cell (Human Umbilical Vein Cells (HUVEC))
Negative control
IgG was used as negative control in ChIP.
Specification
Human Umbilical Vein Cells (HUVEC)
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaledehyde final concent
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaledehyde final concent
Positive control
Transcriptional start site (TSS) of specified genes.
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: sonication buffer for ChIP
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Submitted Oct 16 2017