Overview

  • Product name

  • Description

    Rabbit polyclonal to INO80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human INO80 aa 1400-1450. The exact sequence is proprietary. NP_060023.1
    Database link: Q9ULG1

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245619 was affinity purified using an epitope specific to INO80 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.
    • Tissue specificity

      According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 1 DBINO domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Domain

      The DBINO region is involved in binding to DNA.
    • Cellular localization

      Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.
    • Information by UniProt
    • Database links

    • Alternative names

      • DNA helicase INO80 antibody
      • hINO80 antibody
      • homolog of yeast INO80 antibody
      • INO80 antibody
      • INO80 complex subunit A antibody
      • INO80 homolog antibody
      • INO80_HUMAN antibody
      • INO80A antibody
      • INOC1 antibody
      • KIAA1259 antibody
      • Putative DNA helicase INO80 complex homolog 1 antibody
      see all

    Images

    • INO80 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245619 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using another anti-INO80 antibody at 1 μg/ml.

      Lane 1: ab245619 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

       

    References

    ab245619 has not yet been referenced specifically in any publications.

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