Anti-INO80C antibody (ab151046)
Key features and details
- Rabbit polyclonal to INO80C
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INO80C antibody -
Description
Rabbit polyclonal to INO80C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment, corresponding to a sequence from Human INO80C.
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Positive control
- WB: HEL and transfected HEK293-T whole cell lysate; ICC: U-2 OS whole cells; IHC-P: Human kidney, testits and skeletal muscle tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab151046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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| WB | Use a concentration of 0.04 - 4 µg/ml. Predicted molecular weight: 21 kDa. |
Target
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Relevance
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 125476 Human
- Entrez Gene: 291737 Rat
- SwissProt: Q6PI98 Human
- SwissProt: Q5BJY3 Rat
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Alternative names
- C18orf37 antibody
- FLJ38183 antibody
- hIes6 antibody
see all
Images
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Western blot - Anti-INO80C antibody (ab151046)All lanes : Anti-INO80C antibody (ab151046) at 4 µg/ml
Lane 1 : Control (only vector transfected) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : INO80C over-expression (co-expressed with C-terminal myc-DDk tag) HEK-293T whole cell lysate
Predicted band size: 21 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling INO80C at the nucleoli fibrilar center with ab151046 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human kidney tissue labeling INO80C of tubules cells with ab151046 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-INO80C antibody (ab151046)Anti-INO80C antibody (ab151046) at 4 µg/ml + HEL (human bone marrow erythroleukemia cell line) whole cell lysate
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human testis tissue labeling INO80C at the nuclues of seminiferous ducts with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human skeletal muscle tissue labeling INO80C of myocytes with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)This image is courtesy of an anonymous Abreviewab151046 staining INO80C in Mouse paraffin-fixed tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in Goat serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal (1/125) was used as the secondary antibody. Red - INO80C, Blue (DAPI) - nuclei.
Protocols
Datasheets and documents
References (0)
ab151046 has not yet been referenced specifically in any publications.
