Anti-INO80C antibody (ab151046)
Key features and details
- Rabbit polyclonal to INO80C
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INO80C antibody -
Description
Rabbit polyclonal to INO80C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment, corresponding to a sequence from Human INO80C.
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Positive control
- WB: HEL and transfected HEK293-T whole cell lysate; ICC: U-2 OS whole cells; IHC-P: Human kidney, testits and skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| WB |
Use a concentration of 0.04 - 4 µg/ml. Predicted molecular weight: 21 kDa.
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| Notes |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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WB
Use a concentration of 0.04 - 4 µg/ml. Predicted molecular weight: 21 kDa. |
Target
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Relevance
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 125476 Human
- Entrez Gene: 291737 Rat
- SwissProt: Q6PI98 Human
- SwissProt: Q5BJY3 Rat
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Alternative names
- C18orf37 antibody
- FLJ38183 antibody
- hIes6 antibody
see all
Images
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Western blot - Anti-INO80C antibody (ab151046)All lanes : Anti-INO80C antibody (ab151046) at 4 µg/ml
Lane 1 : Control (only vector transfected) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : INO80C over-expression (co-expressed with C-terminal myc-DDk tag) HEK-293T whole cell lysate
Predicted band size: 21 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling INO80C at the nucleoli fibrilar center with ab151046 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human kidney tissue labeling INO80C of tubules cells with ab151046 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-INO80C antibody (ab151046)Anti-INO80C antibody (ab151046) at 4 µg/ml + HEL (human bone marrow erythroleukemia cell line) whole cell lysate
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human testis tissue labeling INO80C at the nuclues of seminiferous ducts with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human skeletal muscle tissue labeling INO80C of myocytes with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)This image is courtesy of an anonymous Abreviewab151046 staining INO80C in Mouse paraffin-fixed tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in Goat serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal (1/125) was used as the secondary antibody. Red - INO80C, Blue (DAPI) - nuclei.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151046 has not yet been referenced specifically in any publications.