Anti-iNOS antibody (ab3523)
Key features and details
- Rabbit polyclonal to iNOS
- Suitable for: ICC/IF, IHC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-iNOS antibody
See all iNOS primary antibodies -
Description
Rabbit polyclonal to iNOS -
Host species
Rabbit -
Specificity
This antibody detects iNOS. It does not detect other NOS isoforms. By western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS. By western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanIHC Human
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Immunogen
Synthetic peptide corresponding to Mouse iNOS aa 1-100.
Database link: P29477 -
Positive control
- ICC: A549, NIH/3T3 cells. IHC-P: Human heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
Human
|
| IHC |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
1/50.
|
| IHC |
1/20.
|
| Notes |
|---|
|
ICC/IF
1/50. |
|
IHC
1/20. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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Database links
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- Unigene: 709191 Human
- Unigene: 2893 Mouse
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Alternative names
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
Images
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Immunohistochemistry - Anti-iNOS antibody (ab3523)
Immunohistochemistry analysis of human heart tissue stained for iNOS without (negative control) or using ab3523 at 1/200 dilution overnight at 4°C in a humidified chamber, followed biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor.
To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)Immunofluorescence analysis of A549 (human lung carcinoma cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)Immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (199)
ab3523 has been referenced in 199 publications.
- Ahmad N et al. Imperatorin suppresses IL-1ß-induced iNOS expression via inhibiting ERK-MAPK/AP1 signaling in primary human OA chondrocytes. Int Immunopharmacol 85:106612 (2020). PubMed: 32450530
- Miguel-Jiménez S et al. Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation? Int J Mol Sci 21:N/A (2020). PubMed: 32197481
- Zhao JM et al. Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis. AMB Express 10:57 (2020). PubMed: 32198574
- Wang J et al. Suberoylanilide hydroxamic acid alleviates orthotopic liver transplantation-induced hepatic ischemia-reperfusion injury by regulating the AKT/GSK3ß/NF-?B and AKT/mTOR pathways in rat Kupffer cells. Int J Mol Med 45:1875-1887 (2020). PubMed: 32236599
- McKelvey KJ et al. Temporal and spatial modulation of the tumor and systemic immune response in the murine Gl261 glioma model. PLoS One 15:e0226444 (2020). PubMed: 32240177
