Overview

  • Product name

    Anti-iNOS antibody [EPR16630]
    See all iNOS primary antibodies
  • Description

    Rabbit monoclonal [EPR16630] to iNOS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse iNOS aa 1-250. The exact sequence is proprietary.
    Database link: P29477

  • Positive control

    • WB: RAW 264.7 treated with 0.1 µg/ml LPS for 6 hours whole cell lysate. IP: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/80.
WB 1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).

Target

  • Function

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • Tissue specificity

    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • Sequence similarities

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Images

  • All lanes : Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 µg/ml LPS for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell extract with ab205529 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab205529 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract 10ug (Input).

    Lane 2: ab205529 IP in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205529 in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

     

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

References

This product has been referenced in:

  • Gong H  et al. Hippocampal Mrp8/14 signaling plays a critical role in the manifestation of depressive-like behaviors in mice. J Neuroinflammation 15:252 (2018). Read more (PubMed: 30180864) »
See 1 Publication for this product

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