Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16630] to iNOS
- Suitable for: IP, WB
- Reacts with: Mouse
Product nameAnti-iNOS antibody [EPR16630]
See all iNOS primary antibodies
DescriptionRabbit monoclonal [EPR16630] to iNOS
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse iNOS aa 1-250. The exact sequence is proprietary.
Database link: P29477
- WB: RAW 264.7 treated with 0.1 µg/ml LPS for 6 hours whole cell lysate. IP: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab205529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).|
FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Tissue specificityExpressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
- Information by UniProt
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
All lanes : Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 µg/ml LPS for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 1 second
Blocking/Dilution buffer: 5% NFDM/TBST.
iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell extract with ab205529 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab205529 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract 10ug (Input).
Lane 2: ab205529 IP in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205529 in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
ab205529 has been referenced in 1 publication.
- Gong H et al. Hippocampal Mrp8/14 signaling plays a critical role in the manifestation of depressive-like behaviors in mice. J Neuroinflammation 15:252 (2018). PubMed: 30180864