Immunohistochemistry (Frozen sections) abreview for Anti-iNOS antibody [EPR16635]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Melanoma infiltrated tissue)
Permeabilization
No
Specification
Melanoma infiltrated tissue
Blocking step
(agent) for 10 minute(s) · Concentration: 0.25% · Temperature: 23°C
Fixative
Acetone

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% Human Serum in TBS
Dilution
1/50

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
The antibody produced diffuse stain across entire tissue, but also produced bright staining patterns, which could be genuine.

Abcam user community

Verified customer

Submitted Oct 25 2016

Sign up