Western blot abreview for Anti-iNOS antibody [EPR16635]

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide gel)
Loading amount
20 µg
Treatment
UV B 100mJ/cm2
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 130 kDa

Additional data

Additional Notes
lane 1 - non treated control lane 2 - UV B treated control (100mJ/cm2)

Mr. Jeong-Yong Park

Verified customer

Submitted Mar 15 2019

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