Product nameAnti-iNOS antibody [EPR16635-ChiM-IgG2b]
See all iNOS primary antibodies
DescriptionMouse monoclonal [EPR16635-ChiM-IgG2b] to iNOS
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Human iNOS aa 1-200. The exact sequence is proprietary.
Database link: P35228
- WB: RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours. ICC-IF: Raw264.7 cells untreated and LPS treated.
This mouse antibody has been engineered from a RabMAb parent antibody (ab178945). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab210823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Tissue specificityExpressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
- Information by UniProt
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
ab210823 stained in Raw264.7 cells. Untreated and LPS treated (1ug/ml, 24 hours) cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210823 at 5µg/ml overnight at +4°C then detected with an Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). ab206369 (Rabbit monoclonal [EPR16774] to beta Tubulin Alexa Fluor® 594) was used as a counterstaining at a 1/200 dilution for 1hour at room temperature (pseudo-colored red). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Lane 1 : Anti-iNOS antibody [EPR16635-ChiM-IgG2b] (ab210823) at 5 µg
Lane 2 : Anti-iNOS antibody [EPR16635] (ab178945) at 5 µg
All lanes : RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours
Lysates/proteins at 10 µg per lane.
Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Lane 2 : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab210823 (lane 1) and ab178945 (lane 2) overnight at 4°C. Antibody binding was detected using an anti-mouse (lane 1) and anti-rabbit (lane 2) antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab210823 has not yet been referenced specifically in any publications.