Overview

  • Product name
    Anti-iNOS antibody [K13-A]
    See all iNOS primary antibodies
  • Description
    Rabbit monoclonal [K13-A] to iNOS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to a region of Human iNOS.

  • Epitope
    Antibody recognizes the epitope located between Ser1118 - Gly1129
  • Positive control
    • Mouse brain tissue lysate - total protein (ab30151) can be used as a positive control in WB. Raw264.7 whole cell lysate (LPS treated)

Properties

Applications

Our Abpromise guarantee covers the use of ab136918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 131 kDa. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.

Target

  • Function
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • Tissue specificity
    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • Sequence similarities
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Images

  • All lanes : Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

    Lane 1 : Raw264.7 Whole Cell Lysate
    Lane 2 : Raw264.7 Whole Cell Lysate (LPS stimulated)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anit Rabbit IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 131 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 36 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

  • Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + iNOS Overexpression Lysate (Human) at 0.1 µg

    Secondary
    Goat anti Rabbit IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 131 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

  • Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 131 kDa

References

This product has been referenced in:
  • Kozijn AE  et al. Human C-reactive protein aggravates osteoarthritis development in mice on a high-fat diet. Osteoarthritis Cartilage 27:118-128 (2019). Read more (PubMed: 30248505) »
  • P Kwesiga M  et al. Investigative Study on Nitric Oxide Production in Human Dermal Fibroblast Cells under Normal and High Glucose Conditions. Med Sci (Basel) 6:N/A (2018). Read more (PubMed: 30423993) »
See all 7 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-9 of 9 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Sep 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer pH6
Permeabilization
No
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% Normal Buffered Formalin

Abcam user community

Verified customer

Submitted Aug 16 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 27 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% buffered normal formalin

Abcam user community

Verified customer

Submitted Apr 12 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Apr 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered normal formain

Abcam user community

Verified customer

Submitted Mar 30 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 04 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat aorta)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - PBS-Triton X 0.1%
Specification
Rat aorta
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde

Michael Auster

Verified customer

Submitted Jun 22 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
PBS BSA 1% NGS 5% as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Sample
Human Tissue sections (blood vessels)
Specification
blood vessels
Permeabilization
Yes - saponine
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 30 2014

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