Recombinant Anti-iNOS antibody [SP126] (ab115819)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.com/ps/products/115/ab115819/Images/ab115819-5-AntiiNOS-antibody-SP126-IHC-Human-lung-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP126] to iNOS
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-iNOS antibody [SP126]
See all iNOS primary antibodies -
Description
Rabbit monoclonal [SP126] to iNOS -
Host species
Rabbit -
Specificity
We have preliminary internal testing data to indicate this antibody showed no staining in untreated mouse tissue sample by IHC-P
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Tested applications
Suitable for: IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung and lung carcinoma tissue. Flow Cyt (Intra): RAW 264.7 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
SP126 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/100.
Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. We have preliminary internal testing data to indicate this antibody showed no staining in untreated mouse tissue sample by IHC-P |
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
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IHC-P
1/100. Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. We have preliminary internal testing data to indicate this antibody showed no staining in untreated mouse tissue sample by IHC-P |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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Database links
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 709191 Human
see all -
Alternative names
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml).
Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6.0.
Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.com/ps/products/115/ab115819/Images/ab115819-4-AntiiNOS-antibody-SP126-IHC-Human-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml).
Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6.0.
Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain.
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![Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.com/ps/products/115/ab115819/Images/ab115819-330589-anti-inos-antibody-sp126-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] (ab115819)Flow cytometry analysis of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 0.1ug/ml Lipopolysaccharides for 24hours labeling iNOS with purified ab115819 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.com/ps/products/115/ab115819/Images/ab115819-301171-anti-inos-antibody-sp126-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.com/ps/products/115/ab115819/Images/ab115819-127968-anti-inos-antibody-sp126-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] (ab115819)Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.
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![Anti-iNOS antibody [SP126] (ab115819)](https://www.abcam.com/ps/products/115/ab115819/Images/ab115819-6-benefits-of-recombinant-antibodies.png)
Anti-iNOS antibody [SP126] (ab115819)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab115819 has been referenced in 6 publications.
- Ma WH et al. Protective effects of GHK-Cu in bleomycin-induced pulmonary fibrosis via anti-oxidative stress and anti-inflammation pathways. Life Sci 241:117139 (2020). PubMed: 31809714
- Xiao F et al. Platelets and Regulatory T Cells May Induce a Type 2 Immunity That Is Conducive to the Progression and Fibrogenesis of Endometriosis. Front Immunol 11:610963 (2020). PubMed: 33381124
- Zavidij O et al. Single-cell RNA sequencing reveals compromised immune microenvironment in precursor stages of multiple myeloma. Nat Cancer 1:493-506 (2020). PubMed: 33409501
- Tan AHY et al. Lysine-Specific Histone Demethylase 1A Regulates Macrophage Polarization and Checkpoint Molecules in the Tumor Microenvironment of Triple-Negative Breast Cancer. Front Immunol 10:1351 (2019). PubMed: 31249575
- Ottaiano A et al. Cetuximab, irinotecan and fluorouracile in fiRst-line treatment of immunologically-selected advanced colorectal cancer patients: the CIFRA study protocol. BMC Cancer 19:899 (2019). PubMed: 31500586
- Huang MZ et al. Aspirin eugenol ester attenuates oxidative injury of vascular endothelial cells by regulating NOS and Nrf2 signalling pathways. Br J Pharmacol 176:906-918 (2019). PubMed: 30706438