Recombinant
RabMAb

Recombinant Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)

Overview

  • Product name

    Anti-iNOS antibody [SP126] - BSA and Azide free
    See all iNOS primary antibodies
  • Description

    Rabbit monoclonal [SP126] to iNOS - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Mouse iNOS aa 1100 to the C-terminus. The exact sequence is proprietary. Near the C-terminus.
    Database link: P29477

  • Positive control

    • IHC-P: Human Lung, Human lung, and Human lung carcinoma tissue; FC: Raw264.
  • General notes

    Ab239990 is the carrier-free version of ab115819. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239990 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab239990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature.

 

Target

  • Function

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • Tissue specificity

    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • Sequence similarities

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115819)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling iNOS with Purified ab115819 at 1/100 dilution (0.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115819)
  • Flow cytometry analysis of Raw264.7 cells treated with 0.1ug/ml Lipopolysaccharides for 24hours labeling iNOS with purified ab115819 at 1/200 dilution (0.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115819).

  • Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115819).

  • Formalin-fixed, paraffin-embedded human lung stained for iNOS using ab115819 at a dilution of 1/100 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115819).

References

ab239990 has not yet been referenced specifically in any publications.

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