Overview

  • Product name

    Inosine Quantification Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    0.1 nmol/well
  • Assay time

    0h 40m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Inosine Quantification Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.

  • Notes

    Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.

     

  • Platform

    Microplate reader

Properties

Images

  • Inosine standard curve generated using this kit protocol.

Protocols

References

ab126286 has not yet been referenced specifically in any publications.

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