Product nameAnti-INPP4A antibody [EP3425(2)]
See all INPP4A primary antibodies
DescriptionRabbit monoclonal [EP3425(2)] to INPP4A
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human INPP4A aa 50-150. The exact sequence is proprietary.
- Fetal brain and Jurkat cell lysates. Human brain tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|ICC||1/100 - 1/250.|
FunctionCatalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate.
Tissue specificityIsoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain.
PathwaySignal transduction; phosphatidylinositol signaling pathway.
Sequence similaritiesBelongs to the inositol-3,4-bisphosphate 4-phosphatase family.
Contains 1 C2 domain.
- Information by UniProt
- 107kDa antibody
- 4-bisphosphate 4-phosphatase antibody
- 9630012D15 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Human fœtal brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE. Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 104 kDa why is the actual band size different from the predicted?
ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.