Overview

  • Product name
    Anti-INPP4A antibody [EP3425(2)]
    See all INPP4A primary antibodies
  • Description
    Rabbit monoclonal [EP3425(2)] to INPP4A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human INPP4A aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Fetal brain and Jurkat cell lysates. Human brain tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP3425(2)
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function
      Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate.
    • Tissue specificity
      Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain.
    • Pathway
      Signal transduction; phosphatidylinositol signaling pathway.
    • Sequence similarities
      Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
      Contains 1 C2 domain.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • 107kDa antibody
      • 4-bisphosphate 4-phosphatase antibody
      • 9630012D15 antibody
      • D130048C09Rik antibody
      • Inositol polyphosphate 4 phosphatase type I 107kDa antibody
      • Inositol polyphosphate 4 phosphatase type I antibody
      • Inositol polyphosphate 4-phosphatase type I antibody
      • INP4A_HUMAN antibody
      • INPP4 antibody
      • Inpp4a antibody
      • OTTHUMP00000203138 antibody
      • OTTHUMP00000203139 antibody
      • OTTHUMP00000203141 antibody
      • OTTHUMP00000225217 antibody
      • R74740 antibody
      • TVAS1 antibody
      • Type I inositol 3 4 bisphosphate 4 phosphatase antibody
      • Type I inositol 3,4 bisphosphate 4 phosphatase antibody
      • Type I inositol-3 antibody
      see all

    Images

    • Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
      Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: Human fœtal brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE.  Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
      Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
      All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 110 kDa
      Observed band size: 104 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)
      ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Daste F  et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). Read more (PubMed: 28923975) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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