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  1. Link

    inpp4a-antibody-ep34252-ab109622.pdf

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Signal Transduction Signaling Pathway Lipid Signaling Lipid Phosphatases
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-INPP4A antibody [EP3425(2)] (ab109622)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
  • Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)
  • Anti-INPP4A antibody [EP3425(2)] (ab109622)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3425(2)] to INPP4A
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-INPP4A antibody [EP3425(2)]
    See all INPP4A primary antibodies
  • Description

    Rabbit monoclonal [EP3425(2)] to INPP4A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal brain and Jurkat cell lysates. Human brain tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP3425(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Phosphatases

Associated products

  • Alternative Versions

    • Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate.
  • Tissue specificity

    Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain.
  • Pathway

    Signal transduction; phosphatidylinositol signaling pathway.
  • Sequence similarities

    Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
    Contains 1 C2 domain.
  • Target information above from: UniProt accession Q96PE3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3631 Human
    • Entrez Gene: 269180 Mouse
    • Entrez Gene: 80849 Rat
    • Omim: 600916 Human
    • SwissProt: Q96PE3 Human
    • SwissProt: Q9EPW0 Mouse
    • SwissProt: Q62784 Rat
    • Unigene: 469386 Human
    • Unigene: 5294 Mouse
    • Unigene: 11215 Rat
    see all
  • Alternative names

    • 107kDa antibody
    • 4-bisphosphate 4-phosphatase antibody
    • 9630012D15 antibody
    • D130048C09Rik antibody
    • Inositol polyphosphate 4 phosphatase type I 107kDa antibody
    • Inositol polyphosphate 4 phosphatase type I antibody
    • Inositol polyphosphate 4-phosphatase type I antibody
    • INP4A_HUMAN antibody
    • INPP4 antibody
    • Inpp4a antibody
    • OTTHUMP00000203138 antibody
    • OTTHUMP00000203139 antibody
    • OTTHUMP00000203141 antibody
    • OTTHUMP00000225217 antibody
    • R74740 antibody
    • TVAS1 antibody
    • Type I inositol 3 4 bisphosphate 4 phosphatase antibody
    • Type I inositol 3,4 bisphosphate 4 phosphatase antibody
    • Type I inositol-3 antibody
    see all

Images

  • Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
    Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: Human fœtal brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE.  Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
    Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
    All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110 kDa
    Observed band size: 104 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)

    ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-INPP4A antibody [EP3425(2)] (ab109622)
    Anti-INPP4A antibody [EP3425(2)] (ab109622)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab109622? Please let us know so that we can cite the reference in this datasheet.

ab109622 has been referenced in 2 publications.

  • Wang H  et al. Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway. J Biol Chem 295:1091-1104 (2020). PubMed: 31831620
  • Daste F  et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). PubMed: 28923975

Customer reviews and Q&As

Show All Reviews Q&A
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Western blot abreview for Anti-INPP4A antibody [EP3425(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
10 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Feb 05 2021

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