Recombinant Anti-INPP4A antibody [EP3425(2)] (ab109622)
![Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-269757-anti-inpp4a-antibody-ep34252-western-blot.jpg "Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3425(2)] to INPP4A
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-INPP4A antibody [EP3425(2)]
See all INPP4A primary antibodies -
Description
Rabbit monoclonal [EP3425(2)] to INPP4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human INPP4A aa 50-150. The exact sequence is proprietary.
-
Positive control
- Fetal brain and Jurkat cell lysates. Human brain tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3425(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab109622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
Target
-
Function
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate. -
Tissue specificity
Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain. -
Pathway
Signal transduction; phosphatidylinositol signaling pathway. -
Sequence similarities
Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
Contains 1 C2 domain. - Information by UniProt
-
Database links
- Entrez Gene: 3631 Human
- Entrez Gene: 269180 Mouse
- Entrez Gene: 80849 Rat
- Omim: 600916 Human
- SwissProt: Q96PE3 Human
- SwissProt: Q9EPW0 Mouse
- SwissProt: Q62784 Rat
- Unigene: 469386 Human
see all -
Alternative names
- 107kDa antibody
- 4-bisphosphate 4-phosphatase antibody
- 9630012D15 antibody
see all
Images
-
Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Human fœtal brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE. Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-122567-anti-inpp4a-antibody-ep34252-western-blot.jpg)
Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 104 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-122566-anti-inpp4a-antibody-ep34252-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-4-benefits-of-recombinant-antibodies.png)
Anti-INPP4A antibody [EP3425(2)] (ab109622)
Protocols
Datasheets and documents
References (1)
ab109622 has been referenced in 1 publication.
- Daste F et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). PubMed: 28923975
