Recombinant Anti-INPP4A antibody [EP3425(2)] (ab109622)
![Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-269757-anti-inpp4a-antibody-ep34252-western-blot.jpg "Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3425(2)] to INPP4A
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-INPP4A antibody [EP3425(2)]
See all INPP4A primary antibodies -
Description
Rabbit monoclonal [EP3425(2)] to INPP4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Fetal brain and Jurkat cell lysates. Human brain tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3425(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
Target
-
Function
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate. -
Tissue specificity
Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain. -
Pathway
Signal transduction; phosphatidylinositol signaling pathway. -
Sequence similarities
Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
Contains 1 C2 domain. - Information by UniProt
-
Database links
- Entrez Gene: 3631 Human
- Entrez Gene: 269180 Mouse
- Entrez Gene: 80849 Rat
- Omim: 600916 Human
- SwissProt: Q96PE3 Human
- SwissProt: Q9EPW0 Mouse
- SwissProt: Q62784 Rat
- Unigene: 469386 Human
see all -
Alternative names
- 107kDa antibody
- 4-bisphosphate 4-phosphatase antibody
- 9630012D15 antibody
see all
Images
-
Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Human fœtal brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE. Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-122567-anti-inpp4a-antibody-ep34252-western-blot.jpg)
Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 104 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-122566-anti-inpp4a-antibody-ep34252-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.com/ps/products/109/ab109622/Images/ab109622-4-benefits-of-recombinant-antibodies.png)
Anti-INPP4A antibody [EP3425(2)] (ab109622)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab109622 has been referenced in 2 publications.
- Wang H et al. Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway. J Biol Chem 295:1091-1104 (2020). PubMed: 31831620
- Daste F et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). PubMed: 28923975