Overview

  • Product name
    Anti-INPP4B antibody [EPR3108Y]
    See all INPP4B primary antibodies
  • Description
    Rabbit monoclonal [EPR3108Y] to INPP4B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human INPP4B aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O15327

  • Positive control
    • MCF7, fetal skeletal muscle, fetal heart lysates and muscle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3108Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
IHC-P 1/50.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate.
    • Tissue specificity
      Widely expressed with highest levels occurring in the skeletal muscle and heart.
    • Pathway
      Signal transduction; phosphatidylinositol signaling pathway.
    • Sequence similarities
      Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
      Contains 1 C2 domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Inositol polyphosphate 4 phosphatase, type II antibody
      • 4-bisphosphate 4-phosphatase antibody
      • Inositol polyphosphate 4 phosphatase type II 105kDa antibody
      • Inositol polyphosphate 4-phosphatase type II antibody
      • INP4B_HUMAN antibody
      • Inpp4b antibody
      • MGC132014 antibody
      • Type II inositol 3,4 bisphosphate 4 phosphatase antibody
      • Type II inositol-3 antibody
      see all

    Images

    • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human squamous cell carcinoma tissue sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with ab81269 (1/50) for 25 minutes at room temperature.

      INPP4B positive expression shows strong cytoplasmic staining. Magnification: X40.

    • All lanes : Anti-INPP4B antibody [EPR3108Y] (ab81269) at 1/5000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Fetal skeletal muscle lysate
      Lane 3 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 107 kDa
      Observed band size: 107 kDa

    • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human squamous cell carcinoma tissue samples sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with ab81269 (1/50) for 25 minutes at room temperature.

      INPP4B negative expression. Magnification: X40.

    • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human skeletal muscle tissue (negative control) sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with primary antibody substituted for antibody diluent for 25 minutes at room temperature.

      Magnification: X40.

    • Immunohistochemical analysis of paraffin-embedded muscle tissue using ab81269 at 1/50 dilution.

    References

    This product has been referenced in:
    • Muzumdar MD  et al. Survival of pancreatic cancer cells lacking KRAS function. Nat Commun 8:1090 (2017). Read more (PubMed: 29061961) »
    • Asleh-Aburaya K  et al. Basal biomarkers nestin and INPP4b identify intrinsic subtypes accurately in breast cancers that are weakly positive for oestrogen receptor. Histopathology N/A:N/A (2016). Read more (PubMed: 27402148) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human breast cell lines)
    Loading amount
    10 µg
    Specification
    Human breast cell lines
    Gel Running Conditions
    Reduced Denaturing (4-12% NuPAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Damian Yap

    Verified customer

    Submitted Apr 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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