Overview

  • Product name

  • Description

    Rabbit polyclonal to INPP5B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human INPP5B aa 78-178.
    Database link: P32019

  • Positive control

    • IHC-P: Human glioma and pancreas tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab238909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.

Target

  • Function

    Hydrolyzes the calcium-mobilizing second messenger Ins(1,4,5)P3, this is a signal-terminating reaction.
  • Tissue specificity

    Platelets.
  • Sequence similarities

    Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4 antibody
    • 5-trisphosphate 5-phosphatase antibody
    • 5PTase antibody
    • 75 kDa inositol polyphosphate 5 phosphatase antibody
    • 75 kDa inositol polyphosphate-5-phosphatase antibody
    • 75kDa antibody
    • EC 3.1.3.36 antibody
    • I5P2_HUMAN antibody
    • Inositol polyphosphate 5-phosphatase II antibody
    • Inositol polyphosphate-5-phosphatase B antibody
    • Inositol polyphosphate-5-phosphatase, 75kDa antibody
    • INPP5B antibody
    • Inpp5b protein antibody
    • INPP5P antibody
    • MGC65156 antibody
    • MGC71303 antibody
    • OTTHUMP00000004726 antibody
    • OTTHUMP00000004727 antibody
    • OTTHUMP00000004728 antibody
    • Phosphoinositide 5 phosphatase antibody
    • Phosphoinositide 5-phosphatase antibody
    • Type II inositol 1,4,5 trisphosphate 5 phosphatase antibody
    • Type II inositol-1 antibody
    see all

Images

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for INPP5B (Green) using ab238909 at 1/133 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain was DAPI (Blue).

  • Paraffin-embedded human pancreas tissue stained for INPP5B using ab238909 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human glioma tissue stained for INPP5B using ab238909 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab238909 has not yet been referenced specifically in any publications.

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