Key features and details
- Mouse polyclonal to INPP5E
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-INPP5E antibody
See all INPP5E primary antibodies
DescriptionMouse polyclonal to INPP5E
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein, (Human) INPP5E (NP_063945.2, 644 a.a).
- INPP5E transfected 293T cell lysate.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab69696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
FunctionConverts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. Specific for lipid substrates, inactive towards water soluble inositol phosphates.
Tissue specificityDetected in brain, heart, pancreas, testis and spleen.
Involvement in diseaseDefects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) [MIM:213300]. A disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease.
Defects in INPP5E are the cause of mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS) [MIM:610156]. An autosomal recessive disorder characterized by moderate mental retardation, truncal obesity, congenital non-progressive retinal dystrophy, and micropenis in males. The phenotype is similar to Bardet-Biedl syndrome and Cohen syndrome Distinguishing features are the age of onset, the non-progressive nature of the visual impairment, lack of dysmorphic facies, skin or gingival infection, microcephaly, mottled retina, polydactyly, and testicular anomalies.
Sequence similaritiesBelongs to the inositol 1,4,5-trisphosphate 5-phosphatase type IV family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytoskeleton > cilium axoneme. Golgi apparatus > Golgi stack membrane. Peripheral membrane protein associated with Golgi stacks.
- Information by UniProt
- 5-bisphosphate 5-phosphatase antibody
- 72 kDa inositol polyphosphate 5-phosphatase antibody
- Inositol polyphosphate 5 phosphatase antibody
All lanes : Anti-INPP5E antibody (ab69696) at 1/500 dilution
Lane 1 : INPP5E transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
ab69696 has not yet been referenced specifically in any publications.