Key features and details
- Mouse monoclonal [OTI1D4] to INPP5F
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-INPP5F antibody [OTI1D4]
See all INPP5F primary antibodies
DescriptionMouse monoclonal [OTI1D4] to INPP5F
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human INPP5F aa 1-1132. Produced in HEK-293T cells (NP_055752).
MELFQAKDHYILQQGERALWCSRRDGGLQLRPATDLLLAWNPICLGLVEG VIGKIQLHSDLPWWLILIRQKALVGKLPGDHEVCKVTKIAVLSLSEMEPQ DLELELCKKHHFGINKPEKIIPSPDDSKFLLKTFTHIKSNVSAPNKKKVK ESKEKEKLERRLLEELLKMFMDSESFYYSLTYDLTNSVQRQSTGERDGRP LWQKVDDRFFWNKYMIQDLTEIGTPDVDFWIIPMIQGFVQIEELVVNYTE SSDDEKSSPETPPQESTCVDDIHPRFLVALISRRSRHRAGMRYKRRGVDK NGNVANYVETEQLIHVHNHTLSFVQTRGSVPVFWSQVGYRYNPRPRLDRS EKETVAYFCAHFEEQLNIYKKQVIINLVDQAGREKIIGDAYLKQVLLFNN SHLTYVSFDFHEHCRGMKFENVQTLTDAIYDIILDMKWCWVDEAGVICKQ EGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPEQPLPVKCNRI YQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYL NRFKDAYRQAVIDLMQGIPVTEDLYSIFTKEKEHEALHKENQRSHQELIS QLLQSYMKLLLPDDEKFHGGWALIDCDPSLIDATHRDVDVLLLLSNSAYY VAYYDDEVDKVNQYQRLSLENLEKIEIGPEPTLFGKPKFSCMRLHYRYKE ASGYFHTLRAVMRNPEEDGKDTLQCIAEMLQITKQAMGSDLPIIEKKLER KSSKPHEDIIGIRSQNQGSLAQGKNFLMSKFSSLNQKVKQTKSNVNIGNL RKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENTGVMDKVQAESDGD MSSDNDSYHSDEFLTNSKSDEDRQLANSLESVGPIDYVLPSCGIIASAPR LGSRSQSLSSTDSSVHAPSEITVAHGSGLGKGQESPLKKSPSAGDVHILT GFAKPMDIYCHRFVQDAQNKVTHLSETRSVSQQASQERNQMTNQVSNETQ SESTEQTPSRPSQLDVSLSATGPQFLSVEPAHSVASQKTPTSASSMLELE TGLHVTPSPSESSSSRAVSPFAKIRSSMVQVASITQAGLTHGINFAVSKV QKSPPEPEIINQVQQNELKKMFIQCQTRIIQI
Database link: Q9Y2H2
- IHC-P: Human pancreas tissue. WB: HEK-293T cell lysates transfected with pCMV6-ENTRY INPP5F cDNA for 48 hrs.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 128 kDa.|
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.5 before commencing with IHC staining protocol.
RelevanceThe protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants have been observed, but most of them are not thought to be protein coding. There are three isoforms.
- cI-27 antibody
- hSAC2 antibody
- Inositol polyphosphate 5 phosphatase F antibody
Paraffin-embedded human pancreas tissue stained for INPP5F using ab236391 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-INPP5F antibody [OTI1D4] (ab236391) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysates transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T cell lysates transfected with pCMV6-ENTRY INPP5F cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 128 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236391 has not yet been referenced specifically in any publications.