Key features and details
- Suitable for: Blocking
Product nameInsig1 peptide
Our Abpromise guarantee covers the use of ab126817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Insig1 antibody (ab70784)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA
- CL 6
FunctionMediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
Tissue specificityExpressed in all tissues tested with highest expression in the liver.
Sequence similaritiesBelongs to the INSIG family.
modificationsUbiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab126817 has not yet been referenced specifically in any publications.