Overview

  • Product name

    Anti-INSL3 antibody [EPR14834(2)]
    See all INSL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14834(2)] to INSL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human INSL3 aa 1-100. The exact sequence is proprietary.
    Database link: P51460

  • Positive control

    • WB: Human and Mouse testis lysates. IP: Human testis whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14834(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • Function

    Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
  • Tissue specificity

    Expressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast.
  • Involvement in disease

    Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO) [MIM:219050]; also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • INSL 3 antibody
    • INSL3 antibody
    • INSL3_HUMAN antibody
    • Insulin like 3 antibody
    • insulin like peptide, Leydig cell-specific antibody
    • insulin-like 3 (Leydig cell) antibody
    • Insulin-like 3 A chain antibody
    • Ley IL antibody
    • Ley-I-L antibody
    • leydig insulin -like hormone antibody
    • Leydig insulin like peptide antibody
    • Leydig insulin-like peptide antibody
    • prepro-INSL3 antibody
    • Relaxin like factor antibody
    • Relaxin-like factor antibody
    • relaxin-like factor b antibody
    • RLF antibody
    • RLNL antibody
    see all

Images

  • All lanes : Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Human testis lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
    Lane 7 : A431 (Human epidermoid carcinoma) whole cell lysate
    Lane 8 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    INSL3 is expressed in prenatal and postnatal Leydig cells. Human fetal heart, kidney, spleen, NCCIT, A431 & Jurkat cells represent negative controls in this experiment.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human testis whole cell lysate 10 µg (Input). Lane 2: ab199536 IP in Human testis whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab199536 has not yet been referenced specifically in any publications.

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