Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14834(2)] to INSL3
- Suitable for: IP, WB
- Reacts with: Mouse, Human
Product nameAnti-INSL3 antibody [EPR14834(2)]
See all INSL3 primary antibodies
DescriptionRabbit monoclonal [EPR14834(2)] to INSL3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human INSL3 aa 1-100. The exact sequence is proprietary.
Database link: P51460
- WB: Human and Mouse testis lysates. IP: Human testis whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab199536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
FunctionSeems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
Tissue specificityExpressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast.
Involvement in diseaseDefects in INSL3 seems to be a cause of cryptorchidism (CRYPTO) [MIM:219050]; also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low.
Sequence similaritiesBelongs to the insulin family.
- Information by UniProt
- INSL 3 antibody
- INSL3 antibody
- INSL3_HUMAN antibody
All lanes : Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Human testis lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
Lane 7 : A431 (Human epidermoid carcinoma) whole cell lysate
Lane 8 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 minutes
INSL3 is expressed in prenatal and postnatal Leydig cells. Human fetal heart, kidney, spleen, NCCIT, A431 & Jurkat cells represent negative controls in this experiment.
Blocking/Dilution buffer: 5% NFDM/TBST.
INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human testis whole cell lysate 10 µg (Input). Lane 2: ab199536 IP in Human testis whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
ab199536 has not yet been referenced specifically in any publications.