Overview

  • Product name

    Anti-INSL3 antibody [EPR20747]
    See all INSL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20747] to INSL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human INSL3 aa 1-100. The exact sequence is proprietary.
    Database link: P51460

  • Positive control

    • IHC-P: Human testis, ovary and Leydig tumor tissues. IP: Human testis lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20747
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
  • Tissue specificity

    Expressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast.
  • Involvement in disease

    Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO) [MIM:219050]; also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • INSL 3 antibody
    • INSL3 antibody
    • INSL3_HUMAN antibody
    • Insulin like 3 antibody
    • insulin like peptide, Leydig cell-specific antibody
    • insulin-like 3 (Leydig cell) antibody
    • Insulin-like 3 A chain antibody
    • Ley IL antibody
    • Ley-I-L antibody
    • leydig insulin -like hormone antibody
    • Leydig insulin like peptide antibody
    • Leydig insulin-like peptide antibody
    • prepro-INSL3 antibody
    • Relaxin like factor antibody
    • Relaxin-like factor antibody
    • relaxin-like factor b antibody
    • RLF antibody
    • RLNL antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling INSL3 with ab224643 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Leydig cells of human testis (PMID: 19329805). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling INSL3 with ab224643 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in thecal cells of human ovary (PMID: 21138583). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human Leydig tumor tissue labeling INSL3 with ab224643 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human Leydig tumor cells (PMID: 25609776). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • INSL3 was immunoprecipitated from 0.35 mg of human testis lysate with ab224643 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224643 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human testis lysate 10 µg (Input). 

    Lane 2: ab224643 IP in human testis lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224643 in human testis lysate.

    Exposure time: 3 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab224643 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224643.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up