Key features and details
- Rabbit polyclonal to INSL4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-INSL4 antibody
DescriptionRabbit polyclonal to INSL4
Specificityab75061 detects endogenous levels of total INSL4 protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide derived from internal region of human INSL4.
- Extracts from HT-29 cells and A549 cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75061 was purified using an epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab75061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 15 kDa.|
|ICC/IF||1/500 - 1/1000.|
FunctionMay play an important role in trophoblast development and in the regulation of bone formation.
Tissue specificityExpressed in placenta, uterus and in fetal perichondrium. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells.
Sequence similaritiesBelongs to the insulin family.
Developmental stageHighly expressed in the early placenta. Expression of epil peptides in the villous cytotrophoblast is different from that displayed by the syncytiotrophoblast. In fetal tissues it was identified in the perichondrium of all four limbs, vertebrae, and ribs. It was abundant in interbone ligaments.
- Information by UniProt
- early placenta insulin like peptide (EPIL) antibody
- Early placenta insulin like peptide A chain antibody
- Early placenta insulin like peptide antibody
ab75061, at 1/500 dilution, staining of A549 cells using Immunofluorescence analysis.
1) Without immunising peptide
2) With immunising peptide
All lanes : Anti-INSL4 antibody (ab75061) at 1/500 dilution
Lane 1 : extracts from HT-29 cells
Lane 2 : extracts from HT-29 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 15 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
ab75061 has not yet been referenced specifically in any publications.