Recombinant
RabMAb

Recombinant Anti-INSM1 antibody [EPR12044(B)] (ab170876)

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Overview

  • Product name

    Anti-INSM1 antibody [EPR12044(B)]
    See all INSM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12044(B)] to INSM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human INSM1 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01101

  • Positive control

    • Human cerebellum, Fetal brain, Human glioma and Human fetal thymus lysates, U87-MG cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Relevance

      INSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.

    • Cellular localization

      Nuclear

    • Database links

      • Alternative names

        • IA1 antibody
        • Insulinoma associated protein 1 antibody
        • Zinc finger protein IA1 antibody

      Images

      • Immunoprecipitation - Anti-INSM1 antibody [EPR12044(B)] (ab170876)
        Immunoprecipitation - Anti-INSM1 antibody [EPR12044(B)] (ab170876)

        Western blot analysis on immunoprecipitation pellet from fetal brain lysate labeling INSM1 using ab170876 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Immunocytochemistry/ Immunofluorescence - Anti-INSM1 antibody [EPR12044(B)] (ab170876)
        Immunocytochemistry/ Immunofluorescence - Anti-INSM1 antibody [EPR12044(B)] (ab170876)

        Immunofluorescent staining of U87-MG cells labeling INSM1 using ab170876 at a 1/100 dilution.

      • Western blot - Anti-INSM1 antibody [EPR12044(B)] (ab170876)
        Western blot - Anti-INSM1 antibody [EPR12044(B)] (ab170876)
        All lanes : Anti-INSM1 antibody [EPR12044(B)] (ab170876) at 1/1000 dilution

        Lane 1 : Human cerebellum lysate
        Lane 2 : Fetal brain lysate
        Lane 3 : Human glioma lysate
        Lane 4 : Human fetal thymus lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 53 kDa

      References

      This product has been referenced in:

      • Wang L  et al. Targeted deletion of Insm2 in mice result in reduced insulin secretion and glucose intolerance. J Transl Med 16:297 (2018). Read more (PubMed: 30359270) »
      See 1 Publication for this product

      Publishing research using ab170876? Please let us know so that we can cite the reference in this datasheet.

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