Anti-INSM1 antibody [EPR12044(B)] (ab170876)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-INSM1 antibody [EPR12044(B)]
See all INSM1 primary antibodies -
DescriptionRabbit monoclonal [EPR12044(B)] to INSM1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human INSM1 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: Q01101 -
Positive control
- Human cerebellum, Fetal brain, Human glioma and Human fetal thymus lysates, U87-MG cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12044(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab170876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
Target
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RelevanceINSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 3642 Human
- Entrez Gene: 53626 Mouse
- Entrez Gene: 366210 Rat
- Omim: 600010 Human
- SwissProt: Q01101 Human
- SwissProt: Q63ZV0 Mouse
- Unigene: 89584 Human
- Unigene: 379070 Mouse
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Alternative names
- IA1 antibody
- Insulinoma associated protein 1 antibody
- Zinc finger protein IA1 antibody
Images
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![Immunoprecipitation - Anti-INSM1 antibody [EPR12044(B)] (ab170876)](https://www.abcam.com/ps/products/170/ab170876/Images/ab170876-171240-ab170876-3.jpg)
Immunoprecipitation - Anti-INSM1 antibody [EPR12044(B)] (ab170876)Western blot analysis on immunoprecipitation pellet from fetal brain lysate labeling INSM1 using ab170876 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-INSM1 antibody [EPR12044(B)] (ab170876)](https://www.abcam.com/ps/products/170/ab170876/Images/ab170876-171239-ab170876-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-INSM1 antibody [EPR12044(B)] (ab170876)Immunofluorescent staining of U87-MG cells labeling INSM1 using ab170876 at a 1/100 dilution.
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![Western blot - Anti-INSM1 [EPR12044(B)] antibody (ab170876)](https://www.abcam.com/ps/products/170/ab170876/Images/ab170876-171238-ab170876.jpg)
Western blot - Anti-INSM1 [EPR12044(B)] antibody (ab170876)All lanes : Anti-INSM1 antibody [EPR12044(B)] (ab170876) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Fetal brain lysate
Lane 3 : Human glioma lysate
Lane 4 : Human fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 53 kDa
Protocols
Datasheets and documents
References
ab170876 has not yet been referenced specifically in any publications.
