Anti-Insulin + Proinsulin antibody [D3E7] (ab8305)

Submit a review Q&A (17)References (1)

Key features and details

  • Mouse monoclonal [D3E7] to Insulin + Proinsulin
  • Suitable for: ELISA, IHC-Fr
  • Reacts with: Mouse, Rat, Cow, Human, Pig
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

HRP

Overview

  • Product name

    Anti-Insulin + Proinsulin antibody [D3E7]
    See all Insulin + Proinsulin primary antibodies

  • Description

    Mouse monoclonal [D3E7] to Insulin + Proinsulin

  • Host species

    Mouse

  • Specificity

    Kd for this antibody is 6.3 x 10-8M. This antibody is specific for both insulin and proinsulin

  • Tested applications

    Suitable for: ELISA, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig

  • Immunogen

    Full length native protein (purified). Native human insulin

  • General notes


    Detection of insulin and proinsulin

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Target

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab8305? Please let us know so that we can cite the reference in this datasheet.

ab8305 has been referenced in 1 publication.

  • Criscimanna A  et al. Duct cells contribute to regeneration of endocrine and acinar cells following pancreatic damage in adult mice. Gastroenterology 141:1451-62, 1462.e1-6 (2011). IHC-Fr ; Mouse . PubMed: 21763240

Customer reviews and Q&As

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1-10 of 17 Abreviews or Q&A

Question

What is the immunogene of ab8305. I did not see it on the datasheet.

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Answer

Thank you for your e-mail. I would like to confirm that the immunogen was purified human insulin.

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Question

I am trying to use this antibody in a Western blot and I am having some difficulty in determining if the antibody is recognizing insulin. Can you tell me how the ELISA testing was conducted, for example? Did you use both recombinant proinsulin and insulin?

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Answer

Thank you for your enquiry. This antibody was tested along with ab8304 and ab28063 in sandwich ELISAs against recombinant proinsulin and insulin. ab8305 and ab8304 are directed to different antigenic determinants. The Kd for ab8304 is 8,1 x 10-8M. The Kd for ab8305 is 6,3 x 10-8M. The antibodies were used for detection of rat/mouse insulin/proinsulin in two-site enzyme immunoassays and IHC. We recommend to use ab8304 for coating and ab8305 for labeling. The antibodies have not been tested in Western blot. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Customer ask whether ab8305 can identify human recombinant insulin purified from E. coli as a positive control in ELISA? Thank you

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Answer

Thank you for your enquiry. This product has not been tested for use against recombinant human insulin. Ab8305 has only been tested with purified human insulin and proinsulin. If you have any further questions then please get back in touch with us.

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Question

Customer ask whether ab8305 can identify human recombinant insulin purified from E. coli as a positive control in ELISA? Thank you

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Answer

Thank you for your enquiry. This product has not been tested for use against recombinant human insulin. Ab8305 has only been tested with purified human insulin and proinsulin. If you have any further questions then please get back in touch with us.

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Question

The customer needs to know if the antibody has identified Insulin I or Insulin II (in IF with mouse). He has already used the antibody, with sucess, but needs more information now. If you can not tell him which insulin it has identified, do you know the sequence of the immunogen? Thanks for your help.

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Answer

Thank you for your enquiry. The antibody reacts with whole molecule of mouse insulin and proinsulin. The antibody is working in immunohistochemistry on frozen sections. As immunogen we have used native human insulin.

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Question

I'm using the above product ordered from your company, could you tell me the ratio (percentage) of cross-reactivity with human? I need that sort of information urgently for further use.

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Answer

Thank you for your enquiry. Our anti-rat/mouse insulin/proinsulin recognizes rat/mouse insulin with the same efficacy as human insulin or proinsulin.

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Question

Are you able to provide any details about the immunogen used for ab8305 (amino acid sequence)? Thanks!

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Answer

The immunogen used was purified human insulin.

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Question

I want to use Insulin/Proinsulin antibody ab8305 for intracellular staining for FACS. I read the review from Kathy James regarding immunofluorescence. Did she use this antibody for FACS? I tried to read the protocol she used but the link did not work.

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Answer

The customer did not use this antibody in FACS for their review.

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Question

I 'm going to do ELISA using Insulin/Proinsulin antibody (ab8305) [D3E7] and ab6728 Rabbit polyclonal to Mouse IgG H&L (HRP)in order to detect rat insulin, are they suitable for that?

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Answer

Thank you for your enquiry. Ab8305 has been tested for application in ELISA and shown to cross-react with Rat. Ab6728 is a compatible secondary for ab8305. So yes, they should be suitable for your interests. If you have any more questions, please contact us again.

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Question

We want to perform a immunohistochemistry staining on cells cultured on a slide using your anti-insulin/Proinsulin ab8305 antibody. We looked on your website but could not find an ideal dilution for this use (we will be detecting using a biotinylated anti-mouse secondary and then FITC-avidin). Do you have a dilution ideal for this application?

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Answer

Thank you for your enquiry. All the information we have regarding ab8305 is located on the online datasheet. We do not have an ideal dilution for IHC. I suggest that you consult the IHC general protocol section which may be helpful. If you do end up using this antibody for IHC, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

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1-10 of 17 Abreviews or Q&A

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