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    insulin--proinsulin-antibody-d3e7-ab8305.pdf

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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like
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Anti-Insulin + Proinsulin antibody [D3E7] (ab8305)

  • Datasheet
  • SDS
Submit a review Q&A (17)References (1)

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Key features and details

  • Mouse monoclonal [D3E7] to Insulin + Proinsulin
  • Reacts with: Mouse, Rat, Cow, Human, Pig
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
Biotin Anti-Insulin + Proinsulin antibody [D3E7] (ab20756)

View more associated products

Overview

  • Product name

    Anti-Insulin + Proinsulin antibody [D3E7]
    See all Insulin + Proinsulin primary antibodies
  • Description

    Mouse monoclonal [D3E7] to Insulin + Proinsulin
  • Host species

    Mouse
  • Specificity

    Kd for this antibody is 6.3 x 10-8M. This antibody is specific for both insulin and proinsulin
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig
  • General notes


    Detection of insulin and proinsulin

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    Detection of insulin and proinsulin
  • Clonality

    Monoclonal
  • Clone number

    D3E7
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Biotin Anti-Insulin + Proinsulin antibody [D3E7] (ab20756)
    • HRP Anti-Insulin + Proinsulin antibody [D3E7] (ab28063)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Target

  • Relevance

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Defects in insulin are the cause of familial hyperproinsulinemia.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 280829 Cow
    • Entrez Gene: 3630 Human
    • Entrez Gene: 16333 Mouse
    • Entrez Gene: 16334 Mouse
    • Entrez Gene: 24505 Rat
    • Omim: 176730 Human
    • SwissProt: P01317 Cow
    • SwissProt: P01308 Human
    • SwissProt: P01315 Pig
    see all
  • Alternative names

    • ILPR antibody
    • ins antibody
    • Insulin antibody
    • IRDN antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab8305? Please let us know so that we can cite the reference in this datasheet.

ab8305 has been referenced in 1 publication.

  • Criscimanna A  et al. Duct cells contribute to regeneration of endocrine and acinar cells following pancreatic damage in adult mice. Gastroenterology 141:1451-62, 1462.e1-6 (2011). IHC-Fr ; Mouse . PubMed: 21763240

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 17 Abreviews or Q&A

Question

What is the immunogene of ab8305. I did not see it on the datasheet.

Read More

Abcam community

Verified customer

Asked on Oct 31 2006

Answer

Thank you for your e-mail. I would like to confirm that the immunogen was purified human insulin.

Read More

Abcam Scientific Support

Answered on Nov 02 2006

Question

I am trying to use this antibody in a Western blot and I am having some difficulty in determining if the antibody is recognizing insulin. Can you tell me how the ELISA testing was conducted, for example? Did you use both recombinant proinsulin and insulin?

Read More

Abcam community

Verified customer

Asked on Mar 01 2006

Answer

Thank you for your enquiry. This antibody was tested along with ab8304 and ab28063 in sandwich ELISAs against recombinant proinsulin and insulin. ab8305 and ab8304 are directed to different antigenic determinants. The Kd for ab8304 is 8,1 x 10-8M. The Kd for ab8305 is 6,3 x 10-8M. The antibodies were used for detection of rat/mouse insulin/proinsulin in two-site enzyme immunoassays and IHC. We recommend to use ab8304 for coating and ab8305 for labeling. The antibodies have not been tested in Western blot. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 02 2006

Question

Customer ask whether ab8305 can identify human recombinant insulin purified from E. coli as a positive control in ELISA? Thank you

Read More

Abcam community

Verified customer

Asked on Jan 27 2005

Answer

Thank you for your enquiry. This product has not been tested for use against recombinant human insulin. Ab8305 has only been tested with purified human insulin and proinsulin. If you have any further questions then please get back in touch with us.

Read More

Abcam Scientific Support

Answered on Jan 28 2005

Question

Customer ask whether ab8305 can identify human recombinant insulin purified from E. coli as a positive control in ELISA? Thank you

Read More

Abcam community

Verified customer

Asked on Jan 27 2005

Answer

Thank you for your enquiry. This product has not been tested for use against recombinant human insulin. Ab8305 has only been tested with purified human insulin and proinsulin. If you have any further questions then please get back in touch with us.

Read More

Abcam Scientific Support

Answered on Jan 28 2005

Question

The customer needs to know if the antibody has identified Insulin I or Insulin II (in IF with mouse). He has already used the antibody, with sucess, but needs more information now. If you can not tell him which insulin it has identified, do you know the sequence of the immunogen? Thanks for your help.

Read More

Abcam community

Verified customer

Asked on Jun 18 2004

Answer

Thank you for your enquiry. The antibody reacts with whole molecule of mouse insulin and proinsulin. The antibody is working in immunohistochemistry on frozen sections. As immunogen we have used native human insulin.

Read More

Abcam Scientific Support

Answered on Jun 21 2004

Question

I'm using the above product ordered from your company, could you tell me the ratio (percentage) of cross-reactivity with human? I need that sort of information urgently for further use.

Read More

Abcam community

Verified customer

Asked on Mar 17 2004

Answer

Thank you for your enquiry. Our anti-rat/mouse insulin/proinsulin recognizes rat/mouse insulin with the same efficacy as human insulin or proinsulin.

Read More

Abcam Scientific Support

Answered on Mar 19 2004

Question

Are you able to provide any details about the immunogen used for ab8305 (amino acid sequence)? Thanks!

Read More

Abcam community

Verified customer

Asked on Dec 09 2003

Answer

The immunogen used was purified human insulin.

Read More

Abcam Scientific Support

Answered on Dec 10 2003

Question

I want to use Insulin/Proinsulin antibody ab8305 for intracellular staining for FACS. I read the review from Kathy James regarding immunofluorescence. Did she use this antibody for FACS? I tried to read the protocol she used but the link did not work.

Read More

Abcam community

Verified customer

Asked on Nov 18 2003

Answer

The customer did not use this antibody in FACS for their review.

Read More

Abcam Scientific Support

Answered on Nov 19 2003

Question

I 'm going to do ELISA using Insulin/Proinsulin antibody (ab8305) [D3E7] and ab6728 Rabbit polyclonal to Mouse IgG H&L (HRP)in order to detect rat insulin, are they suitable for that?

Read More

Abcam community

Verified customer

Asked on Nov 17 2003

Answer

Thank you for your enquiry. Ab8305 has been tested for application in ELISA and shown to cross-react with Rat. Ab6728 is a compatible secondary for ab8305. So yes, they should be suitable for your interests. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Nov 17 2003

Question

We want to perform a immunohistochemistry staining on cells cultured on a slide using your anti-insulin/Proinsulin ab8305 antibody. We looked on your website but could not find an ideal dilution for this use (we will be detecting using a biotinylated anti-mouse secondary and then FITC-avidin). Do you have a dilution ideal for this application?

Read More

Abcam community

Verified customer

Asked on Oct 21 2003

Answer

Thank you for your enquiry. All the information we have regarding ab8305 is located on the online datasheet. We do not have an ideal dilution for IHC. I suggest that you consult the IHC general protocol section which may be helpful. If you do end up using this antibody for IHC, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Read More

Abcam Scientific Support

Answered on Oct 21 2003

1-10 of 17 Abreviews or Q&A

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