Overview

  • Product name
    Anti-Insulin antibody [8E2] (HRP)
    See all Insulin primary antibodies
  • Description
    Mouse monoclonal [8E2] to Insulin (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Specificity
    Kass for this antibody is 6.0 x 108M-1. It cross-reacts with human proinsulin. No cross-reaction with free C-peptide.
  • Tested applications
    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Full length native protein (purified) (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab24455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [3A6] to Insulin (ab1965). Can be used as Detection antibody with recommended pair.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease
    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the insulin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

References

ab24455 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Question

This email has 2 sections:
A recap of the complaint
The respondent’s survey results (section-by-section)
Recap of the complaint
---------------------------------------------------------------------------------------------------------------------
Date(s) submitted: January 6, 2012
Catalog number(s): 24455
Product(s): Mouse monoclonal [8E2] to Insulin (HRP)
CCEID: *****
Survey results
---------------------------------------------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. I do not remember

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.No
Q. How did you feel about being given protocol advice?
A.
Q. Did the product work successfully after following the advice?
A.
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.
A.
A.
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.No
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Completely unsatisfied
Q. Could we have done anything to better resolve the problem?
A.Change supplier
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please provide a phone number or email address where we can reach you.
A.j.dutreuil@adocia.com
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.le lot pour lequel j'ai contacté le service client fonctionnait mal, le lot suivant est pire encore. Sans autres commentaires... Je cherche une autre solution chez un autre fournisseur.

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Answer

Merci d'avoir pris le temps de répondre à notre questionnaire.

Je suis désolé de ne pas avoir pu résoudre correctement le problème que vous avez rencontré avec l'anticorps anti-insuline ab24455 lorsque vous m'avez contacté début janvier. Je suis également désolé de constaterque vous avez perdu confiance en ce produit et peut-être plus généralement en la qualité des produits Abcam.

Nous avons à présent en stock un nouveau lot de cet anticorps ab24455.

Seriez-vous d'accord pour que je vous envoie une unité gratuite de ce lot afin que vous le testiez?

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Answer

Thank you for contacting us.

In our https://www.abcam.com/index.html?pageconfig=resource&rid=11422 we recommend carbonate/bicarbonate buffer at pH 9.6. However, the capture antibody can also be diluted with PBS, pH 7.4, and the HRP-conjugated detection MAb with PBS, 0.05% Tween-20.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Application
ELISA
Sample
Mouse Purified protein (Insulin standards)
Specification
Insulin standards
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 37°C
Type
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Oct 10 2012

Answer

Thank you for your reply.
Please keep in mind that mouse has not been tried and we do not know if and how well the antibodies will work with this species.
I'll pass the lot numbers to the lab.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your phone call.
I have contacted the lab for more information about antibody dilutions and for a detailed protocol, and will let you know when I hear back.
Below, please find the discount codes for trying these antibodies with mouse samples.

DISCOUNT CODE: xxx for ab1965
DISCOUNT CODE: xxx for ab24455
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab1965 and ab24455 in mouse. Each code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Question
Answer

For ab24455 DISCOUNT CODE: ***

For ab1965 DISCOUNT CODE: ***

Expiration date: December 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab1965 and ab24455 for useon mouse samples. Each code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for using each antibody inmouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for yoiru enquiry.

I can confirm that these two antibodies have been tested together in Sandwich ELISA system. The storage instructions can be found on the on-line product datasheets. We normally suggest our customers using the products within 6 months (Abpromise) to make sure that we can offer our full guarantee even though after proper aliquotting they can be active for months or for several years depending on the storage temperature.

Standard ELISA, sELISA can be carried out on special ELISA plates (96-well) but the size of the plates really depends on the customer's need. Thermo Scientific, Nunc, BD Biosciences sell different variety of ELISA plates.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Merci pour votre réponse.

J'ai mis en place l'envoi de 4 unités de ab24455. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Merci de me faire part de vos résultats avec ce nouveau lot. Si il n'y a pas d'amélioration je crains que nous devrons changer radicalement de stratégiepourl'approvisionnement de ce produit.

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Answer

Je suis vraiment désolé que le dernier lot GR73820 ne soit pas, une fois de plus, de bonne qualité.

C'est malheureusement le seul lot que nous avons actuellement en stock. Je pensais en vous contactant la semaine dernière que vous aviez mentionnédans le questionnairele lot précédentpour lequel vous m'aviez contacté.

J'ai de nouveau signalé le problème à notre collaborateur qui produit cet anticorps. Je vais m'assurer que les dispositions nécessaires vont être prises afin que ce problème ne se reproduise pas une troisième fois. Cette situation ne correspond aucunement aux standards de qualité d'Abcam.

Je vous contacterai de nouveau dès que j'auraiplus d'informations. J'espère qu'un nouveau lot de bonne qualité sera prochainement disponible, je vous enverrai alors 4 unités gratuitesen remplacementdes 4 tubes du lot GR73820.

Toutes mes excuses pour ce désagrément et merci d'avancepour votre patience.

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Answer

Thank you for your response.

You have raised an excellent point that I will bring to our datasheet team. While we do list sandwich ELISA as a different application perhaps we could list the specific ELISA type as we do for IHC. In most cases it would read Indirect ELISA and Direct ELISA. I would like to thank you for this suggestion.

The principles behind the sandwich and indirect ELISAs (which are the application type we guarantee) are very much the same however an sandwich ELISA uses two antibodies against a specific target protein, one as capture on as detection, whereas a normal Indirect ELISA use an antibody against the species as capture and against the target protein as detection. The problems with sandwich are that the two antibodies against the same target protein may interfere with one another leading to poor or no signal. Because of this we only recommend using products that have been tested in sandwich ELISA or are matched pairs.

This information may be found on our website by searching for the protein of interest in the search bar while using the 'primary antibodies' search in the drop down menu. After the results are displayed, further narrow down the field to only those tested in sandwich ELISA by using the filter on the left hand side. In this case I was able to find 2 anti-insulin antibodies tested in sELISA (ab1965 and ab24455) as well as a few for proinsulin.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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