Anti-Insulin antibody [8E2] (HRP) (ab24455)


  • Product name
    Anti-Insulin antibody [8E2] (HRP)
    See all Insulin primary antibodies
  • Description
    Mouse monoclonal [8E2] to Insulin (HRP)
  • Host species
  • Conjugation
  • Specificity
    Kass for this antibody is 6.0 x 108M-1. It cross-reacts with human proinsulin. No cross-reaction with free C-peptide.
  • Tested applications
    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Full length native protein (purified) (Human)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.



Our Abpromise guarantee covers the use of ab24455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [3A6] to Insulin (ab1965). Can be used as Detection antibody with recommended pair.
ELISA Use at an assay dependent dilution.


  • Function
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease
    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the insulin family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all


ab24455 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Merci d'avoir pris le temps de répondre à notre questionnaire.

Je suis désolé de ne pas avoir pu résoudre correctement le problème que vous avez rencontré avec l'anticorps anti-insuline ab24455...

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Thank you for contacting us.

In our we recommend carbonate/bicarbonate buffer at pH 9.6. However, the capture antibody can also be diluted with PBS, pH 7.4, and the HRP-conjug...

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Mouse Purified protein (Insulin standards)
Insulin standards
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 37°C
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Oct 10 2012

Thank you for your reply.
Please keep in mind that mouse has not been tried and we do not know if and how well the antibodies will work with this species.
I'll pass the lot numbers to the lab.

I hope this information is helpful to you....

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Thank you for your phone call.
I have contacted the lab for more information about antibody dilutions and for a detailed protocol, and will let you know when I hear back.
Below, please find the discount codes for trying these antibodies with ...

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For ab24455 DISCOUNT CODE: ***

For ab1965 DISCOUNT CODE: ***

Expiration date: December 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab1965 and ab24455 for useon mouse samples. Each code...

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Thank you for yoiru enquiry.

I can confirm that these two antibodies have been tested together in Sandwich ELISA system. The storage instructions can be found on the on-line product datasheets. We normally suggest our customers using the prod...

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Merci pour votre réponse.

J'ai mis en place l'envoi de 4 unités de ab24455. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les d&eacu...

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Je suis vraiment désolé que le dernier lot GR73820 ne soit pas, une fois de plus, de bonne qualité.

C'est malheureusement le seul lot que nous avons actuellement en stock. Je pensais en vous contactant la semaine derni&eg...

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Thank you for your response.

You have raised an excellent point that I will bring to our datasheet team. While we do list sandwich ELISA as a different application perhaps we could list the specific ELISA type as we do for IHC. In most cases ...

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