Overview

  • Product name

  • Description

    Guinea pig polyclonal to Insulin
  • Host species

    Guinea pig
  • Tested applications

    Suitable for: IHC-P, ICC, IHC-Frmore details
  • Species reactivity

    Reacts with: Human, Pig
  • Immunogen

    Immunogen (human insulin) was conjugated with R-phycoerythrin (R-PE) as a carrier protein.

  • Positive control

    • Human pancreas tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab195956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Streptavidin-biotin or polymer secondary antibody detection recommended.

ICC Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/100.

Streptavidin-biotin or polymer secondary antibody detection recommended.

Target

  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Images

  • ab195956 staining Insulin in pig pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 22°C; antigen retrieval was by heat mediation in Tris-EDTA, pH 9.0. Samples were incubated with the primary antibody (1/100 in 10% serum in PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-guinea pig IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Gurlo T  et al. Pregnancy in human IAPP transgenic mice recapitulates beta cell stress in type 2 diabetes. Diabetologia 62:1000-1010 (2019). Read more (PubMed: 30852627) »
  • Cai C  et al. Loss-of-function myostatin mutation increases insulin sensitivity and browning of white fat in Meishan pigs. Oncotarget 8:34911-34922 (2017). Read more (PubMed: 28432282) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.4% TritonX in TBS
Specification
Pancreas
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Chun Wang

Verified customer

Submitted Nov 22 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 02 2017

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