Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Insulin antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum in PBS
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Guinea pig IgG (H+L) Cross-Adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

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Submitted May 02 2017

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