Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Insulin antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.4% TritonX in TBS
Specification
Pancreas
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Same as blocking buffer
Dilution
1/100

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donke
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The antibody shows good cytosolic staining for mouse pancreatic beta cells. However there also appear to be some unspecific nuclear staining in the acinar tissue.

Chun Wang

Verified customer

Submitted Nov 22 2018

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