• Product name
    Anti-Insulin antibody [E2E3]
    See all Insulin primary antibodies
  • Description
    Mouse monoclonal [E2E3] to Insulin
  • Host species
  • Specificity
    The antibody recognizes the biologically most active forms of insulin on the C terminal end. The antibody labels the cytoplasm of beta cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human, Pig, Monkey
  • Immunogen

    Full length protein corresponding to Pig Insulin.



Our Abpromise guarantee covers the use of ab9569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Staining of formalin-fixed tissue sections requires treating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT.
WB Use at an assay dependent concentration. Used at 1/100 for 1 hr on human fusion protein in transgenic seed (see Abreview sent by Cory L. Nykiforuk for furher information).
ELISA Use at an assay dependent concentration. Recommended for insulin monitoring in sera from patients with diabetes or insulin producing tumors.


  • Function
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease
    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the insulin family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all


  • Human pancreas stained with ab9569.The antibody labels the cytoplasm of ß cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
  • Formalin-fixed, paraffin-embedded monkey pancreas tissue stained for Insulin using ab9569 at 1/800 dilution in immunhistochemical analysis.

    Goat Ani-mouse HRP was used as the secondary antibody.


This product has been referenced in:
  • Tian M  et al. Alternative immunomodulatory strategies for xenotransplantation: CD80/CD86-CTLA4 pathway-modified immature dendritic cells promote xenograft survival. PLoS One 8:e69640 (2013). IHC-P . Read more (PubMed: 23922766) »
  • Nykiforuk CL  et al. Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds. Plant Biotechnol J 4:77-85 (2006). WB ; Human . Read more (PubMed: 17177787) »

See all 2 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Monkey Tissue sections (Pancreas)
Antigen retrieval step
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Nizar Mourad

Verified customer

Submitted Apr 19 2016

Thank you very much for your call today and for your enquiry.

As we discussed, please keep me updated about the results with this antibody. Should you have any trouble with it, I will be happy to set up the testing program for you in retrospe...

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Thank you for your enquiry.
The antibody should be OK. I would recommend using the antibody in an experiment using a positive control sample to make sure it is still active.
Store remainder of antibody at 4 degrees C.
I hope this is help...

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Thank you for contacting us. Because the sequence is conserved between the monomeric and hexameric forms of insulin, it would not be possible to get antibodies that would be specific for one form over the other. The easiest way to tell the difference b...

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Thank you for your inquiry and I apologize for the delay in this reply.

We do not test this antibody in WB in-house. The only information we have about the use of this antibody is from customer data in the form of an Abreview and refere...

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Thank you for your inquiry. Please excuse the delay of my answer, but I am contacting the source of ab8304 and haven't heard back from them yet. But I am happy to confirm that ab9569 will weakly detect non-reduced insulin (needed to load at least...

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According to our records, ab9569 was proving difficult to use in WB and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the dif...

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Thank you for your enquiry. I apologize for the delay in replying to you. It has taken some time to gather all the required information. Sensitivity of antibodies in ELISA assays depends very much on the individual assay procedure and the type o...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and you are largely following an ELISA protocol that we would recommend. There is no reason why these ...

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Western blot
Arabidopsis thaliana Recombinant protein (total seed extract)
total seed extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Cory L Nykiforuk

Verified customer

Submitted Feb 24 2006

1-10 of 13 Abreviews or Q&A


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